WormBase Tree Display for Expr_pattern: Expr3521
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| Expr3521 | Expression_of | Gene | WBGene00005020 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00005020 | ||
| Expression_data | Life_stage | WBls:0000003 | |
| GO_term | GO:0005794 | ||
| Subcellular_localization | The anti-Gal-T2 scFv showed a strong immunofluorescence signal in all cells of early and late embryo specimens, revealing numerous punctate organelles surrounding the nucleus. Nuclei were always devoid of fluorescence and the pattern of anti-Gal-T2 scFv antibody fluorescence is similar to that seen with other examples of worm Golgi proteins. | ||
| Type | Antibody | ||
| Picture | WBPicture0000008770 | ||
| Remark | To determine whether the antibody was against the Gal-T2 protein and not the recombinant tags or putative N- or O-glycan side chains, Western blot analysis against recombinant and deglycosylated Gal-T2 protein preparations were used. ELISA and Western blot analysis showed that the antibody recognized a recombinant Gal-T2 (31 to 330-aa) protein preparation, lacking the purification tags. | ||
| Reference | WBPaper00026630 | ||
| Antibody_info | WBAntibody00000873 |
