WormBase Tree Display for Expr_pattern: Expr3427
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| Expr3427 | Expression_of | Gene | WBGene00004896 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004896 | |||
| Expression_data | Anatomy_term | WBbt:0004017 | Certain | |
| GO_term | GO:0005730 | |||
| GO:0005634 | ||||
| Subcellular_localization | SMU-2::GFP was occasionally observed localized to a subnuclear compartment that probably is the nucleolus because of its morphology and its increased size in ncl-1 mutants, which have enlarged nucleoli. However, nucleolar localization was dependent on the health of the animal. Animals suffering from prolonged exposure to sodium azide showed a dramatic increase in nucleolar localization. Nucleolar localization of SMU-2::HA was rarely observed. SMU-2 probably is not normally localized to the nucleolus. No other consistent pattern of subnuclear localization was observed. | |||
| Type | Reporter_gene | |||
| Pattern | SMU-2::GFP is a nuclear protein. SMU-2::GFP was observed at all stages of development including early embryogenesis and in oocytes. To show that SMU-2 is ubiquitously expressed, embryos and larvae carrying the integrated smu-2::gfp array were costained with the DNA stain DAPI and SMU-2::GFP was observed in essentially every cell that was stained by DAPI. Unlike most transgenes in C. elegans, the smu-2::gfp transgene was expressed in the germ line. To confirm this result with another SMU-2-expressing transgene, a rescuing transgene that placed a HA tag at the very end of the protein were constructed. This construct also led to nuclear expression in all cells, including the germ line. | |||
| Reference | WBPaper00024265 | |||
| Transgene | WBTransgene00001008 |
