WormBase Tree Display for Expr_pattern: Expr3165

Expr3165 Expression_of: Gene: WBGene00003839
    Reflects_endogenous_expression_of: WBGene00003839
  Expression_data: Anatomy_term: WBbt:0003830 Certain
      WBbt:0003831 Certain
      WBbt:0003889 Certain
      WBbt:0003890 Certain
      WBbt:0003995 Certain
      WBbt:0003997 Certain
      WBbt:0003999 Certain
      WBbt:0004003 Certain
    GO_term: GO:0005886
      GO:0097730
  Subcellular_localization: Expressed in cilia and plasma membrane of the chemosensory neurons ADF, AWA, ASH, and ADL.
  Type: Reporter_gene
  Pattern: The immunoreactivity of OCR-2::FLAG and OCR-2(G36E)::FLAG is detected in the cilia and plasma membrane of the chemosensory neurons ADF, AWA, ASH, and ADL.
  Remark: Whereas ocr-2(ak47) animals carrying the OCR-2::FLAG transgene restore tph-1 expression (68% show strong tph-1::gfp in ADF; n = 79), ocr-2(ak47) animals that carry OCR-2(G36E)::FLAG (n = 79) exhibit reduced tph-1::gfp expression as their nontransgenic siblings (n = 235). These data indicate that FLAG-tagged OCR-2 is localized to the cellular sites in which OCR-2 acts and that the G36E substitution does not alter the subcellular localization. These results suggest that the G36E substitution disrupts the function of the OCR-2/OSM-9 channel in the ADF neurons.
  Reference: WBPaper00024918
  Transgene: WBTransgene00028405