WormBase Tree Display for Expr_pattern: Expr3121
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| Expr3121 | Expression_of | Gene | WBGene00003844 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003844 | |||
| Homol | Homol_homol | K11C4:Expr | ||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0005772 | Certain | ||
| WBbt:0006794 | Certain | |||
| Type | Reporter_gene | |||
| Pattern | Substantial fluorescence was noted in the 20 intestinal cells of adult and larval stages. During the embryonic development GFP signals were first seen in eggs that are situated in the uterus close to the vulva, representing embryos at the time of gastrulation, approximately 3 h after fertilisation. pPD95.77CeODC-1 promotes a male-specific GFP expression in parts of the reproductive system. | |||
| Remark | The constructs pPD95.77CeODC(2047), pPD95.77CeAdoMet(3720) and pPD95.77CeSPDS(1687) were generated using the same sense primers as above but the anti sense primers 5'-GGA TCC AAT GTC ACG GCA CAT TTG GAG-3' (ODC-AS1), 5'-CCC GGG AAA GTT GGT GGC AGA CGT GGC-39 (AdoMet-AS1) GGA TCC GTT CAT GGC TTT TCG GAG (SPDS-AS1), respectively, that are located within the first 50 bp of the open reading frames. The constructs pPD95.77CeSPDS(1687), pPD95.77CeAdoMet(3720) and pPD95.77CeODC(2047) exhibited the same expression pattern as pPD95.77CeSPDS-1, pPD95.77CeAdoMet-1 or pPD95.77CeODC-1, indicating that the regions downstream of the translational start site do not contain promoter elements that affect the expression pattern of the three genes. | |||
| Reference | WBPaper00024333 | |||
| Transgene | WBTransgene00028372 |
