WormBase Tree Display for Expr_pattern: Expr3044
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| Expr3044 | Expression_of | Gene | WBGene00002000 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002000 | |||
| Homol | Homol_homol | F58D5:Expr | ||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0004017 | Certain | ||
| GO_term | GO:0005634 | |||
| Subcellular_localization | In all stages, the GFP was nuclear localised despite the absence of a NLS in the vector, implying that sequences within HRP-2 can direct nuclear localisation. While the nuclear fluorescence observed in some tissues such as the gut was largely homogeneous, a punctate pattern of fluorescence was particularly obvious in neuronal cells of larval stages. Both the tissue and subcellular localisation patterns were confirmed by reactivity of an antibody (R64) specific for the N-terminus of HRP-2. Reactivity was observed in all the nuclei of the worm as defined by costaining with DAPI. | |||
| Type | Antibody | |||
| Reporter_gene | ||||
| Pattern | HRP-2 is expressed in all cells of the early embryo commencing between the 28- and 64-cell stages, with staining of the large gut cell nuclei being particularly prominent by the 3-fold stage of development. This ubiquitous pattern of expression continues throughout larval development and into the adult worm. As transgenes are typically poorly expressed in the germline, the gonad was dissected from adult worms and found to be clearly reactive with R64 Ab. Antibody staining also confirmed expression of HRP-2 in the large nuclei of the gut cells. No staining was observed with the preimmune serum. | |||
| Picture | WBPicture0000011824 | |||
| WBPicture0000011825 | ||||
| Reference | WBPaper00024309 | |||
| Transgene | WBTransgene00028317 | |||
| Antibody_info | WBAntibody00000755 |
