WormBase Tree Display for Expr_pattern: Expr2832

Expr2832 Expression_of: Gene: WBGene00001196
    Reflects_endogenous_expression_of: WBGene00001196
  Expression_data: Life_stage: WBls:0000024
      WBls:0000003
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
    Anatomy_term: WBbt:0004017 Certain
    GO_term: GO:0030424
  Subcellular_localization: Expressed in axons of the nerve ring.
  Type: Antibody
  Pattern: Immunolocalization analysis with a polyclonal antibody generated against the carboxyl terminus of EGL-30 generally revealed the areas with highest expression levels. Using an HRP-conjugated secondary antibody and a FITC-conjugated secondary antibody, high expression was observed in axons of the nerve ring. The FITC-conjugated antibody also indicated expression in pharyngeal muscle. Both secondary antibodies revealed expression in sperm, and the FITC-conjugated antibody consistently showed expression in the anal sphincter muscle.
    Weak or occasional expression in other muscle-cell types, sperm, intestine, tonofilaments, and vulval cells indicates the possibility that EGL-30 might have an almost ubiquitous expression pattern.
  Picture: WBPicture0000011812
  Remark: Nonspecific antibody staining was observed in intestinal nuclei using both secondary antibodies and is most likely due to cross-reactivity with an unrelated protein. Western analysis supports this hypothesis since the band that migrates at the position where EGL-30 migrates on an SDS/PAGE gel is present at almost equivalent intensities in protein extracts prepared from egl-30(ad805) mutants compared to wild-type extracts, but the levels of this band increase significantly after heat-shock induction of an egl-30 transgene. When egl-30 was overexpressed from an integrated array (syIs36), an increase in the intensity of staining was observed in the nerve ring and in pharyngeal muscle, but not in intestinal nuclei.
  Reference: WBPaper00006314
  Antibody_info: WBAntibody00000706