WormBase Tree Display for Expr_pattern: Expr2782
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| Expr2782 | Expression_of | Gene | WBGene00004862 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004862 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005300 | Certain | |||
| WBbt:0005319 | Certain | |||
| WBbt:0005753 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0005812 | Certain | |||
| WBbt:0006748 | Certain | |||
| Type | Reporter_gene | |||
| Pattern | sma-9 promoter-driven GFP was expressed in the ventral nerve cord, pharynx and intestine, seam cells, excretory canal, vulva and spermatheca. Expression was observed from L1 to adult stages but not during embryonic development. sma-9 promoter-driven GFP was detected in the lateral seam only from the L1 to the L3 stages, and not in the L4 stage or in the adult. | |||
| Picture | WBPicture0000007575 | |||
| Remark | Different promoter regions do not give rise to the same expression pattern. The 2.8 kb, 5.5 kb and 8.0 kb constructs (upstream of predicted exon 1) show strong fluorescence in all detected tissues except the seam cells. The 1.5 kb construct (5500 bp to 4000 bp relative to predicted exon 1) displays expression in the seam cells, ventral nerve cord and excretory canal only. The 4.0 kb construct (8000 bp to 4000 bp) generates the complete expression pattern, indicating the presence of redundant transcriptional elements. The 5.5 kb and 8.0 kb fragments are identical to the 1.5 kb and 4.0 kb fragments, respectively, except for the addition of sequences from 4000 bp to 1 bp that include the alternative exon 1. The addition of these sequences abolishes expression in the seam cells in these reporters. These results could be due to the presence of a seam-cell specific repressor element between 4000 bp and 1 bp. Alternatively, transcription and splicing in the seam cells might specifically generate the Class B variant that initiates translation in alternative exon 1, which would render the GFP sequences in the 5.5 kb and 8.0 kb constructs out of frame. | |||
| Reference | WBPaper00006346 | |||
| Transgene | WBTransgene00028175 |
