WormBase Tree Display for Expr_pattern: Expr2747
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| Expr2747 | Expression_of | Gene | WBGene00001679 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001679 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000003 | |||
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| Anatomy_term (14) | |||
| GO_term | GO:0005818 | ||
| GO:0016020 | |||
| GO:0005834 | |||
| Subcellular_localization | GPB-1 is detected most strongly at the cell membrane, with staining concentrated at the contact between cells. Interestingly, staining also colocalizes with the asters (arrays of microtubules emanating from the centrosomes) just before and during early cell divisions. | ||
| Type | Antibody | ||
| Pattern | In situ immunohistochemistry with this antiserum shows that GPB-1 is present in early embryos. Diffuse staining can be seen at the 1-cell stage. From the 2-cell stage onward. Up to the beginning of morphogenesis, staining continues at approximately equal levels in most or all cells. Once tissue differentiation occurs, staining is brightest in neuronal cells. In larval and adult stages, GPB-1 expression is seen in most or all neurons, including the nerve ring, the dorsal and ventral nerve cords, and the preanal ganglion. In addition, the somatic gonad, vulva, and hypodermal seam cells have high expression. Other tissues, such as the intestine, pharynx, and body wall muscles, appear to have a low level of expression. GPB-1 is also expressed in the germline. | ||
| Picture | WBPicture0000009498 | ||
| Remark | The specificity of the antibody staining is indicated by the following controls: first, the staining is competed away by preincubation of the antibody with GPB-1 protein and, second, gpb-1 mutant embryos do not exhibit any staining. | ||
| Reference | WBPaper00002525 | ||
| Antibody_info | WBAntibody00000117 |
