WormBase Tree Display for Expr_pattern: Expr2676
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| Expr2676 | Expression_of | Gene | WBGene00004738 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004738 | |||
| Expression_data | Life_stage | WBls:0000003 | ||
| WBls:0000057 | ||||
| Anatomy_term | WBbt:0005175 | Certain | ||
| GO_term | GO:0005634 | |||
| Subcellular_localization | Expressed in nuclei. | |||
| Type | Antibody | Rabbit and Guinea pig polyclonal antibody against amino acid 207479 of SCC-3 was produced using GST-tagged recombinant protein. Rabbit antiserum was used to stain gonads and embryos. | ||
| Pattern | In embryos and in mitotic cells of the gonad, SCC-3 was found dispersed within nuclei. Beginning in the transition zone and most clearly in pachytene, linear structures were highlighted by the antiserum. Double immunostaining for SCC-3 and its putative partner REC-8 showed that the two proteins colocalize on pachytene bivalents. In diplotene to diakinesis, SCC-3 was localized within nuclei but was not detectable on bivalents, although REC-8 outlines the axes of bivalents at these stages. SCC-3 was not detected on condensed mitotic chromosomes. | |||
| Remark | As a control, gonads depleted of SCC-3 were immunostained for SCC-3. Staining was negative in these nuclei, in contrast to histone H1 immunostaining, which was done as a positive control. | |||
| Reference | WBPaper00006112 | |||
| Antibody_info | WBAntibody00000675 | |||
| WBAntibody00000676 |
