WormBase Tree Display for Expr_pattern: Expr2664
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| Expr2664 | Expression_of | Gene | WBGene00003478 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003478 | |||
| Expression_data | Anatomy_term | WBbt:0005792 | Certain | |
| GO_term | GO:0016324 | |||
| Subcellular_localization | MTM-6-GFP fusion protein was observed at high levels in a uniform pattern associated with the apical membrane of the intestinal cell. By contrast, expression of GFP alone, or a GFP fusion protein containing only the MTM-6 FYVE domain, exhibited a diffuse cytosolic expression in the intestine. | |||
| Type | Reporter_gene | |||
| Pattern | Expressed in intestinal cell. | |||
| Remark | Gene_regulation: MTM-6 contains a putative amino-terminal GRAM domain, a PTP catalytic domain, a set interaction domain (SID), and a carboxyl-terminal FYVE domain. The GRAM domain is a novel 70 amino acid domain located at the amino terminus of several MTMs and has been proposed to act as an intracellular protein or lipid-binding signaling module. The SID was identified initially on the basis of its interaction with the SET domain of the Hrx protooncogene and might also mediate protein-protein interactions. Although both MTM-6 and MTM-1 contain putative GRAM and SID domains, only MTM-6 contains a FYVE domain, suggesting that this domain might target MTM-6 to the plasma membrane. However, neither the GRAM nor the FYVE domains are required for apical targeting as MTM-6-GFP fusion proteins lacking either the FYVE or the GRAM domain target to apical membrane. The role the PTP or SID domains could not be assessed because mutants that lack either the PTP or SID domains are not expressed, presumably because this results in the production of an unstable protein. | |||
| Reference | WBPaper00006051 | |||
| Transgene | WBTransgene00028117 |
