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WormBase Tree Display for Expr_pattern: Expr2561

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Name Class

Expr2561Expression_ofGeneWBGene00000413
Reflects_endogenous_expression_ofWBGene00000413
Expression_dataLife_stageWBls:0000003
WBls:0000041
Anatomy_termWBbt:0005784Certain
GO_termGO:0005634
Subcellular_localizationnuclei
TypeAntibodyRabbit polyclonal antibody. Anti-CDT-1 sera were produced in rabbits using purified recombinant His-tagged full-length CDT-1 fusion protein expressed from the pET15b vector. Affinity purification was performed with His-CDT-1 fusion protein linked to activated CL4B Fastflow Sepharose. Affinity-purifiedanti-CDT-1antibodies from two different rabbits gave the same pattern of immunofluorescence staining.
PatternIn adults, CDT-1 is present in germ cell nuclei and is enriched in oocyte nuclei. In early embryos, CDT-1 is localized to chromosomes in mitotic cells during anaphase and early telophase. CDT-1 nuclear staining is not present in S phase, which directly follows mitosis in the early embryo. Anti-CDT-1 staining in embryos is abolished by cdt-1 RNAi, indicating the validity of the staining pattern. Newly hatched larvae lack detectable CDT-1 expression. However, CDT-1 levels are transiently increased in the nuclei of blast cells before their mitotic divisions.
In the wild type, CDT-1 expression began approximately 20 min after hatching in a subset of seam cells, and by 120 min post-hatch all seam cells expressed CDT-1 at high levels. At 130 min post-hatch, the number of seam cells with CDT-1 protein dropped precipitously, and by 150 min post-hatch most seam cells no longer had CDT-1 protein. Significantly, the decrease in CDT-1 levels in S-phase cells occurred in both the nucleus and cytoplasm, indicating that CDT-1 does not undergo subcellular redistribution, but instead is degraded.
ReferenceWBPaper00005912
Antibody_infoWBAntibody00000626