WormBase Tree Display for Expr_pattern: Expr2561
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Expr2561 | Expression_of | Gene | WBGene00000413 | |
---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00000413 | |||
Expression_data | Life_stage | WBls:0000003 | ||
WBls:0000041 | ||||
Anatomy_term | WBbt:0005784 | Certain | ||
GO_term | GO:0005634 | |||
Subcellular_localization | nuclei | |||
Type | Antibody | Rabbit polyclonal antibody. Anti-CDT-1 sera were produced in rabbits using purified recombinant His-tagged full-length CDT-1 fusion protein expressed from the pET15b vector. Affinity purification was performed with His-CDT-1 fusion protein linked to activated CL4B Fastflow Sepharose. Affinity-purifiedanti-CDT-1antibodies from two different rabbits gave the same pattern of immunofluorescence staining. | ||
Pattern | In adults, CDT-1 is present in germ cell nuclei and is enriched in oocyte nuclei. In early embryos, CDT-1 is localized to chromosomes in mitotic cells during anaphase and early telophase. CDT-1 nuclear staining is not present in S phase, which directly follows mitosis in the early embryo. Anti-CDT-1 staining in embryos is abolished by cdt-1 RNAi, indicating the validity of the staining pattern. Newly hatched larvae lack detectable CDT-1 expression. However, CDT-1 levels are transiently increased in the nuclei of blast cells before their mitotic divisions. | |||
In the wild type, CDT-1 expression began approximately 20 min after hatching in a subset of seam cells, and by 120 min post-hatch all seam cells expressed CDT-1 at high levels. At 130 min post-hatch, the number of seam cells with CDT-1 protein dropped precipitously, and by 150 min post-hatch most seam cells no longer had CDT-1 protein. Significantly, the decrease in CDT-1 levels in S-phase cells occurred in both the nucleus and cytoplasm, indicating that CDT-1 does not undergo subcellular redistribution, but instead is degraded. | ||||
Reference | WBPaper00005912 | |||
Antibody_info | WBAntibody00000626 |