WormBase Tree Display for Expr_pattern: Expr2323
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| Expr2323 | Expression_of | Gene | WBGene00000390 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000390 | ||||
| Expression_data | Life_stage | WBls:0000003 | |||
| Anatomy_term | WBbt:0004017 | Certain | |||
| Remark | cdc-42 is ubiquitously expressed throughout embryogenesis. | ||||
| GO_term | GO:0005886 | ||||
| Subcellular_localization | cell boundaries | ||||
| Type | Antibody | Polyclonal antibodies against CDC42Ce was raised and used in indirect immunofluorescence analysis. The affinity-purified antibody against CDC42Ce recognized a single 23-kDa protein in extracts of mixed stage population of C. elegans. The specificity of the antibody was demonstrated by control Western blot analysis with recombinant CDC42Ce/GST protein, which showed no cross-reactivity of the antibodies. | |||
| Pattern | Ubiquitously expressed throughout embryogenesis. However, at the stage when hypodermal cell fusion was occurring CDC42Ce was found to localize with CePAK at the cell boundaries. | ||||
| Picture | WBPicture0000008097 | ||||
| WBPicture0000008099 | |||||
| Remark | No signal was detected using the antibody when it was blocked with its GST fusion protein. | ||||
| Reference | WBPaper00002544 | ||||
| Antibody_info | WBAntibody00000119 |
