WormBase Tree Display for Expr_pattern: Expr2308
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| Expr2308 | Expression_of | Gene | WBGene00003916 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003916 | |||
| Expression_data | Life_stage | WBls:0000038 | ||
| WBls:0000035 | ||||
| WBls:0000057 | ||||
| Anatomy_term | WBbt:0006748 | Certain | ||
| GO_term | GO:0005938 | |||
| Subcellular_localization | PAR-1 distribution was examined from the mid-L3 stage to young adulthood, using AJM-1 as a marker for the identity and position of vulval cells through vulval development. PAR-1 is localized near apical junctions prior to vulval development. This localization is common to other C. elegans epithelial cells. After fate specification and precursor proliferation, PAR-1 is not only found near apical junctions, but it is also associated with the cortex of the vulval cells. Through morphogenesis, PAR-1 gradually undergoes a redistribution from near apical junctions to the basolateral cortex. By young adulthood, PAR-1 is highly enriched in basolateral domains and little, if any, is still found associated with junctions. The PAR-1 distribution was identical using three different sources of antibodies to PAR-1 and, was sensitive to par-1(RNAi). | |||
| Type | Antibody | Anti-PAR-1 polyclonal antibodies. Three antisera to PAR-1 produced identical staining patterns in the vulva of developing larvae. These included antiserum raised against a portion of the linker region(see Expr578) and two antisera raised against overlapping portions of the carboxyl terminus. | ||
| Reference | WBPaper00005653 | |||
| Antibody_info | WBAntibody00000096 | |||
| WBAntibody00000598 |
