WormBase Tree Display for Expr_pattern: Expr2301
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| Expr2301 | Expression_of | Gene | WBGene00006796 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006796 | |||
| Expression_data | Life_stage | WBls:0000041 | ||
| WBls:0000005 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0005784 | Certain | ||
| Type | RT_PCR | |||
| Pattern | Further evidence for differential accumulation was obtained using quantitative real-time PCR analysis (QPCR) with appropriate transcript-specific primers The levels of the rare 7a transcripts increase over 100-fold from early embryos to adulthood. In addition, the QPCR results show that the levels of 7b mRNAs (unclear from the RNA blot because of non-uniform loading) are approximately the same in adult soma and complete adults, indicating that both 7b and 7a mRNAs are present in adult soma. The lower relative levels of 7a mRNAs in complete adults compared with adult soma raises the possibility that 7a mRNAs are not present in the hermaphrodite germline. | |||
| RNA blot analysis of stage-specific total RNA with transcript-specific probes showed different accumulation patterns for the four unc-62 mRNAs. The 1a-containing mRNAs were more abundant than the 1b-containing mRNAs, but their temporal patterns of accumulation appeared similar. Both were present in adult germline, pregastrulation embryos, later embryos and adult soma. The 7a signals were too weak to assess relative abundances at different stages. The 7b-containing mRNAs appeared much more abundant than the 7a RNAs in both embryos and adults. | ||||
| Reference | WBPaper00005629 |
