WormBase Tree Display for Expr_pattern: Expr21
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| Expr21 | Expression_of | Gene | WBGene00006768 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006768 | ||
| Clone | pUL#AL10 | ||
| Expression_data | Life_stage (11) | ||
| Anatomy_term (24) | |||
| Type | Reporter_gene | ||
| Pattern | The last expression component to appear is in certain cells of the somatic gonad. The D-cells of the vulval labia and unidentified cells of the spermathecal structures begin expression in L4, whilst gonadal morphogenesis is ongoing. The D-cells do not express beyond the first oocyte fertilisations (no zygotes are usually visible when these cells are stained), the spermathecal staining lasting slightly longer into adulthood | ||
| The next stage at which expression is evident is during the elongation phase of late embryogenesis when the worm is approximately 2 fold. The nuclei of the M2 motor neurones in the terminal bulb of the pharynx stain strongly. More pharyngeal cells show expression as morphogenesis proceeds until at hatching the two I1 interneurones of the metacorpus, either the e2 or m2 cells of the procorpus, and the m8 muscle cell at the pharyngeal-intestinal boundary can all be seen. This pattern remains through the rest of the life cycle, although the m8 expression is lost during early larval stages | |||
| These early larval stages also see the appearance of expression in the tail region. The nuclei of the anal sphincter cell and 3/4 neuronal cells of the posterior ganglia comprise this regional component of the pattern | |||
| This gene gives rise to a complicated multicomponent developmental expression pattern. Earliest expression is seen during the cleavage stage of embryogenesis, in the clonal descendants of the E blastomere, the founder cell giving rise the whole of the gut of the adult animal. Expression begins in Ea and Ep just after gastrulation, and continues into each of the granddaughters of these two cells. At this stage, the expressing cells clearly outline the emerging form of the gut. This component ends at about the 150/200 cell stage | |||
| Picture | WBPicture0000006547 | ||
| WBPicture0000007161 | |||
| WBPicture0000007162 | |||
| WBPicture0000007163 | |||
| Remark | 50-70 cell embryo(author) = 51-cell embryo(curator). | ||
| early embryo(author) = blastula + gastrulating embryo(curator). | |||
| fragment altered 7/97, at request of IHope | |||
| late embryo(author) = 2-fold embryo(curator). | |||
| life_stage summary : each cell-group has different pattern | |||
| Experiment | Strain | WBStrain00045971 | |
| Reference | WBPaper00002318 | ||
| Transgene | WBTransgene00027898 |
