WormBase Tree Display for Expr_pattern: Expr1941
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| Expr1941 | Expression_of | Gene | WBGene00004960 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004960 | |||
| Expression_data | Life_stage | WBls:0000057 | ||
| Anatomy_term | WBbt:0006798 | Certain | ||
| Type | Antibody | polyclonal rabbit antibody to SPE-6 peptide. | ||
| RT_PCR | ||||
| Western | ||||
| Pattern | spe-6 mRNA could be amplified from fem-3(q23gf) hermaphrodite mRNA (this mutant has hermphrodite soma but makes only sperm germline), but not not from fem-1(hc17ts) hermaphrodite mRNA (mutant hermaphrodites make only oocytes, no sperm); cDNA clone ca01c03 (GenBank accesion nos. AW057333 and AW057136) are from spermatogenesis-specific libraries; SPE-6 protein only detected in extracts from sperm-producing worms (i.e. fem-3(gf), not fem-1(lf) on western blots. | |||
| Remark | Data submitted by Paul Muhlrad, unpublished (Ph.D. dissertation) and cgc5276. Protein_Description: casein kinase 1. WP:CE28799. | |||
| Differential RT-PCR (RNA from fem-3(gf) vs. fem-1(lf) mutants; differential western blot; immunofluorescence staining | ||||
| Reference | WBPaper00005276 | |||
| Antibody_info | WBAntibody00000515 |
