WormBase Tree Display for Expr_pattern: Expr1899
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| Expr1899 | Expression_of | Gene | WBGene00001358 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001358 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| WBls:0000015 | |||
| WBls:0000021 | |||
| Anatomy_term (21) | |||
| Type | Reporter_gene | ||
| Pattern | Expression of the construct is first detected during embryogenesis, at the beginning of morphogenesis. evl-20::gfp reporter is strongly expressed in the migrating hypodermal cells throughout the enclosure process. Shortly after the beginning of elongation, evl-20::gfp is turned on in many developing neurons, where it persists through adulthood. (This pattern reflects zygotic evl-20 expression, and it is possible that the maternally produced endogenous gene product is also present in the early embryo. However, such maternal expression cannot be recapitulated by the reporter construct due to the germline silencing effect.) In larva, the construct is expressed in a subset of developing tissues. The reporter construct is highly expressed in all 22 cells of the vulva in mid L4 stage. No apparent difference in levels of expression between cells adopting primary and secondary vulval fates can be seen. evl-20::gfp expression can first be detected in P5.p to P7.p during the two-cell stage, after the first round of VPCs divisions. GFP expression was observed in the somatic gonad during L2 to L4 stages. The expression was the strongest in the uterus, although it was also detectable in the spermatheca, sheath cells, and the distal tip cells (DTC). Expression of the construct in the vulva and the gonad was undetectable in adult worms. GFP expression is also seen in many cells during male tail development, with the highest levels found in the proctodeum. As mentioned previously, evl-20 is also expressed in most neurons during larval stages and in the adult worm. | ||
| Picture | WBPicture0000009908 | ||
| Reference | WBPaper00005256 | ||
| Transgene | WBTransgene00027743 | ||
| WBTransgene00031652 |
