WormBase Tree Display for Expr_pattern: Expr1869
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| Expr1869 | Expression_of | Gene | WBGene00004085 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004085 | ||
| Expression_data | GO_term | GO:0005737 | |
| GO:0005813 | |||
| Subcellular_localization | Immunostaining using the affinity-purified antibodies revealed that PPH-4.1 localized to centrosomes during mitosis. The signal was first detected as two small dots adjacent to the male pronucleus, corresponding to the centrosomes of sperm asters. The signal became more intense at metaphase through anaphase, concomitantly with enlargement of the asters. The signal at centrosomes then became weaker and dispersed in telophase, and was undetectable in interphase. PPH-4.1 was also present in the cytoplasm, throughout the cell cycle. This cell-cycle-dependent localization of PPH-4.1 was observed in later embryonic cell divisions too. Consistent with the results of RNAi showing that PPH-4.1 is dispensable for female meiotic divisions, PPH-4.1 was not detected at meiotic spindles in fertilized eggs. No signal was detected in pph-4.1(RNAi) embryos that did not form a spindle, further confirming the specificity of the antibody. | ||
| Type | Antibody | Rabbit polyclonal antibodies against the amino-terminal 197 amino acids of PPH-4.1. | |
| Picture | WBPicture0000009032 | ||
| Remark | No detailed description on cellular expression patterns. | ||
| Reference | WBPaper00005225 | ||
| Antibody_info | WBAntibody00000500 |
