WormBase Tree Display for Expr_pattern: Expr16238
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| Expr16238 | Expression_of | Gene | WBGene00002081 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002081 | ||||
| Expression_data | Life_stage | WBls:0000024 | Anatomy_term | WBbt:0004824 | |
| WBbt:0004825 | |||||
| WBbt:0004826 | |||||
| WBbt:0004837 | |||||
| WBbt:0004838 | |||||
| WBbt:0004839 | |||||
| WBbt:0005190 | |||||
| WBbt:0005733 | |||||
| WBbt:0006840 | |||||
| WBls:0000038 | Anatomy_term (22) | ||||
| Anatomy_term (22) | |||||
| Type | Reporter_gene | ||||
| Pattern | We observed low levels of Pina-1::GFP expression in GABAergic motor neurons of both L1 and L4 animals. At the L1 stage, where only DD GABAergic neurons are present, we noted dim fluorescence in DD cell bodies that was obscured by comparatively high levels of Pina-1::GFP epidermal expression. At the L4 stage, somatic fluorescence in GABAergic motor neurons remained dim, but was more clearly discernible in comparison with L1, likely due to decreased Pina-1::GFP expression in the epidermis of L4 stage animals. We noted consistent low levels of expression across both VD and DD GABAergic neurons. We also noted stronger labeling of additional ventral cord motor neurons that were not labeled by the Punc-47::mCherry reporter, suggesting expression of ina-1 in other motor neuron classes. To address this possibility, we generated a transgenic strain co-expressing the Pina-1::GFP reporter with the cholinergic marker Pacr-2::mCherry. Overall, we noted that Pina-1::GFP fluorescence was stronger in cholinergic motor neurons compared with GABAergic motor neurons, and was expressed in only a subset of cholinergic motor neurons at both L1 and L4 stages. At the L4 stage, Pina-1::GFP fluorescence was most consistently observed labeling the B-class cholinergic motor neurons. | ||||
| Reference | WBPaper00057276 | ||||
| Transgene | WBTransgene00033548 |
