WormBase Tree Display for Expr_pattern: Expr15997
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| Expr15997 | Expression_of | Gene | WBGene00007514 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00007514 | ||
| Expression_data | Anatomy_term (12) | ||
| Type | Genome_editing | ||
| Reporter_gene | We used CRISPR/ Cas9 engineering to insert a tagRFP fluorescent protein at the N-terminus of CATP-8. | ||
| Pattern | We found these animals to show widespread, if not ubiquitous expression of CATP-8, including in the epidermis, muscle, pharynx, intestine, and the major neuronal ganglia. Of note, we detected expression in both neurons that were phenotypically affected in catp-8 mutants as well as neurons that were not visibly affected. For example, clear expression of the endogenous tagRFP::CATP-8 reporter was seen in HSN, PVM, and PVD neurons, but also in ALM neurons and others. We found similar expression patterns in transgenic animals expressing GFP under control of the catp-8 5' region immediately upstream of the ATG start codon, where we also saw widespread expression from embryonic stages onwards, including in muscle, intestine, pharynx and other tissues. Collectively, our studies show that catp-8 is widely, if not ubiquitously, expressed albeit at different levels. | ||
| Reference | WBPaper00061583 |
