WormBase Tree Display for Expr_pattern: Expr15685

Expr15685 Expression_of: Gene: WBGene00006604
    Reflects_endogenous_expression_of: WBGene00006604
  Expression_data: Life_stage: WBls:0000038 Anatomy_term: WBbt:0005735
          WBbt:0007850
      WBls:0000035 Anatomy_term: WBbt:0005735
          WBbt:0007850
      WBls:0000041 Anatomy_term: WBbt:0005735
          WBbt:0007850
    Anatomy_term: WBbt:0005735 Certain
        Life_stage: WBls:0000035
          WBls:0000038
          WBls:0000041
      WBbt:0007850 Certain
        Life_stage: WBls:0000035
          WBls:0000038
          WBls:0000041
  Type: Genome_editing
    Reporter_gene
  Pattern: As expected, we observed extensive GFP::TRA-1 fluorescence in multiple tissues of larval and adult hermaphrodites. Surprisingly, however, we also detected significant GFP::TRA-1 in males, confined almost exclusively to the nervous system. In L3 larval males, GFP::TRA-1 was apparent in numerous head neurons, though not in as many as seen in L3 hermaphrodites. This pattern was maintained in L4 males, but, by the time of young adulthood, far fewer neurons contained GFP::TRA-1.Using a pan-neural marker, we quantified the number of GFP::TRA-1-positive neurons in the region spanned by the pharyngeal bulbs. This revealed that tra-1 is expressed in roughly 54 and 49 head neurons in L3 and L4 males, respec- tively, and that this number decreases to 25 in 1-day-old adult males. Using a marker for ciliated sensory neurons, we found that roughly 17 of the tra-1-expressing neurons in this region in L3 males were of this class, and that this number decreased to 9 in 1-day-old adult males. These findings suggest that tra-1 function in the nervous system might not be limited to hermaphrodites, and that any roles it may have in the male soma could be both cell type and stage specific.
  Reference: WBPaper00060018
  Variation: WBVar02148945