WormBase Tree Display for Expr_pattern: Expr1498
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| Expr1498 | Expression_of | Gene | WBGene00004895 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004895 | |||
| Expression_data | Life_stage | WBls:0000002 | ||
| Anatomy_term | WBbt:0004017 | Certain | ||
| WBbt:0005784 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | The protein products were found localized to nuclei. | |||
| Type | Reporter_gene | [smu-1::gfp] translational fusion and [smu-1::HA] translational fusion. smu-1 is full-length in the constructs. smu-1 was fused to either the gene for GFP or sequence encoding three tandem HA tags. The expression patterns of these two full-length rescuing transgenes were analyzed by direct visualization of GFP or by antibody staining with anti-GFP or anti-HA antibodies. | ||
| Pattern | The transgenes were ubiquitously expressed throughout development. In hermaphrodite larvae, expression of the rescuing transgenes was seen in all types of somatic nuclei and in the nuclei of the female germ line. Occasionally neuronal nuclei failed to stain with anti-GFP antibodies in integrated lines containing the rescuing smu-1::gfp transgene. This was a very small proportion of the neurons, and lack of staining in these cases could have been due either to technical aspects of the antibody staining protocol or to incomplete expression of the integrated transgene. Nuclei of the other major tissue types (hypodermis, intestine, and muscle) always stained under the same conditions. In mid- to late-stage embryos, autofluorescing GFP was visible in all or most nuclei. In embryos of less than about 200 cells, it was unable to detect GFP directly, but fixation and staining with anti-GFP antibodies indicated that GFP was present in all of the nuclei of early embryos that were not in the process of dividing. Tiny foci of anti-GFP staining within these nuclei were often seen, but it is not clear whether they represent a pattern of subnuclear localization or are due to fixation or staining artifacts. Obvious foci were not seen in the cells of other developmental stages, although in some cases the fluorescence had a rough or uneven appearance when studied closely. | |||
| Remark | Germ line expression was surprising because most transgenic arrays seem to be subject to germ line silencing. | |||
| Reference | WBPaper00004764 | |||
| Transgene | WBTransgene00000290 |
