WormBase Tree Display for Expr_pattern: Expr14714
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| Expr14714 | Expression_of | Gene | WBGene00009926 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00009926 | ||
| Expression_data | GO_term | GO:0031012 | |
| Subcellular_localization | NOAH-1 and NOAH-2 were distributed as a layer wrapping around the embryos with local differences, consistent with the notion that they are ECM components. Specifically, before the 2-fold stage, NOAH-1_mCH (int) and NOAH-2_GFP(int) were enriched above seam cells and in lines positioned like CeHDs at that stage. At later elongation stages, they displayed a fiber-like pattern in dorso-ventral (DV) cells, consistent with ZP proteins, which are often organized in filaments (Jovine et al., 2005). NOAH-2_GFP(int) displayed a dottier pattern and less obvious circumferential stripes at the 3-fold stage, perhaps because it was only partially functional. When combined, NOAH-1_mCH(int) and NOAH-2_GFP(int) showed colocalization, in particular at the circumferential stripes at late elongation stages. As the circumferential stripes were reminiscent of the actin bundles in the DV epidermal cells, we compared NOAH-1 distribution with that of epidermal actin visualized with a GFP-labeled actin-binding domain (referred to as ABD::GFP) (Gally et al., 2009). Deconvoluted confocal images and fluorescence profile measurement clearly indicated that NOAH-1 was apical to cortical actin, consistent with an extracellular localization. | ||
| Type | Genome_editing | ||
| Reporter_gene | |||
| Reference | WBPaper00051259 | ||
| Variation | WBVar02149381 |
