WormBase Tree Display for Expr_pattern: Expr1427
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| Expr1427 | Expression_of | Gene | WBGene00006743 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006743 | |||
| Expression_data | Life_stage | WBls:0000022 | ||
| WBls:0000020 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0003887 | Certain | ||
| WBbt:0003888 | Certain | |||
| WBbt:0005270 | Certain | |||
| WBbt:0005274 | Certain | |||
| WBbt:0005278 | Certain | |||
| WBbt:0005303 | Certain | |||
| WBbt:0005304 | Certain | |||
| WBbt:0005334 | Certain | |||
| WBbt:0005336 | Certain | |||
| WBbt:0005339 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | nuclei | |||
| Type | Antibody | Polyclonal rabbit antibody against peptide derived from UNC-3. | ||
| Reporter_gene | ||||
| Pattern | Antibodies directed against the C terminus of CeO/E were used to confirm the GFP reporter experiments and examine the expression of CeO/E in early development. Anti-CeO/E staining were observed in 8 nuclei in the 400- to 550-cell embryo, the stage during which the embryonic motor neurons are first formed. The CeO/E-expressing cells were located in positions consistent with motor neurons destined for the ventral cord: initially located on the posterior side close to the periphery of the embryo and subsequently converging toward the ventral midline. By comma stage, the number of CeO/E-expressing cells increased to 16 and these cells became positioned along the presumptive ventral cord, again consistent with their identification as embryonic motor neurons. In late stage embryos and early L1 larvae, 16 cells expressed CeO/E at high levels along the ventral cord, in addition to at least 6 faintly immunoreactive cells also located along the ventral cord in the head and tail regions. | |||
| CeO/E expression was observed in two cells outside of the ventral cord. Using the CeO/E antibody, expression in these cells was first detected in 400 to 550-cell stage in the most anterior region of the embryo. By comma stage, the immunoreactive nuclei were located closer to the ventral cord in a position consistent with that of the ASI amphid neurons. These cells continued to stain with anti-CeO/E antibody and expressed unc-3::GFP throughout development. These cells were confirmed as ASI neurons by staining adult worms expressing unc-3::GFP with DiI. | ||||
| In all lines, expression of unc-3::GFP was first observed in late stage embryos (3-fold) in neurons of the ventral cord. In L1 larvae, 16 cells were counted that expressed unc-3::GFP among the 22 embryonic motor neurons present in the ventral cord at this stage. By late L1 and early L2, when 57 new motor neurons are added to the ventral cord, an increase was observed in the number of GFP-positive cells. In two lines expressing GFP from an extrachromosomal array, there are up to 41 ventral cord motor neurons expressing the reporter at similar intensity. In the integrated line examined, the 23 VA and VB motor neurons expressed GFP at high levels, while 3 strongly staining and up to 11 additional faintly expressing cells were also detected. High levels of expression were maintained in these neurons through the L3 stage, but diminished in later stages. No GFP expression was observed in the ventral cord of a majority of adult worms. | ||||
| The P neuroblast divides to generate hypodermal cells and 3 to 5 neurons, each belonging to a different class of postembryonic ventral cord neurons (VA, VB, VC, VD or AS). During this time, CeO/E staining in the embryonic motor neurons decreased in intensity. More than 40 ventral cord motor neurons stained prominently in L2 and later stages. clusters of 3 to 5 cells were frequently observed along the ventral cord expressing CeO/E. | ||||
| Picture | WBPicture0000007980 | |||
| Remark | CeO/E = unc-3 in this article. | |||
| Reference | WBPaper00003083 | |||
| Transgene | WBTransgene00000272 | |||
| Antibody_info | WBAntibody00000177 |
