WormBase Tree Display for Expr_pattern: Expr1416
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| Expr1416 | Expression_of | Gene | WBGene00006351 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006351 | ||
| Expression_data | Life_stage | WBls:0000002 | |
| Anatomy_term (29) | |||
| GO_term | GO:0005634 | ||
| GO:0005737 | |||
| Subcellular_localization | Both constructs are expressed in nuclei; and a relatively small amount of SUR-5GFP fusion protein from pTG96_1 is detected in cytoplasm. | ||
| Type | Reporter_gene | ||
| Pattern | When transgenic animals carrying pTG96 on an extrachromosomal array (kuEx75) were examined, the fusion protein, as judged by fluorescence of GFP, was observed tightly localized to the nuclei of most cells. SUR-5 appeared to be expressed in the VPCs. Cell types that express this fusion protein include neurons, hypodermis, Pn.p cells, body muscles, many cells of the pharynx, and a few cells of the somatic gonads. Cells that do not display the fluorescence include B, F, K , K.a, K.p, hyp3, the germ line, and the excretory duct cells. In nonmosaic animals, the intensity varies among the cells. The intestinal cells and excretory cells are almost always very bright, whereas neurons are almost always fainter. Uterine cells and many of the cells derived from the M cell are very faint and often difficult to see. The SUR-5GFP fusion proteins are expressed in all stages of C. elegans development. The earliest expression is at the 100- to 150-cell embryonic stage, and the fusion proteins are expressed throughout development from that stage on. The same expression pattern is seen when this array is integrated into one of the chromosomes. | ||
| pTG96_1 is still localized in the nuclei of most cells. The expression pattern is the same as that seen from the array containing pTG96 (with NLS), but the nuclear localization is not as tight, and there appears to be some diffusion of SUR-5GFP proteins from the nucleus to the cytoplasm. | |||
| Remark | Integrated transgenic line not described in the article. | ||
| Reference | WBPaper00003142 | ||
| Transgene | WBTransgene00027255 | ||
| WBTransgene00027256 |
