WormBase Tree Display for Expr_pattern: Expr1402
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| Expr1402 | Expression_of | Gene | WBGene00003559 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003559 | ||
| Expression_data | Life_stage | WBls:0000003 | |
| WBls:0000057 | |||
| GO_term | GO:0005737 | ||
| Subcellular_localization | In 300-cell stage embryos: Immunostaining with mAb D3C2 showed that NCL-1 is present in the cytoplasm of all cells except those of the developing gut. | ||
| In early embryos in which nucleoli are not detectable in wild-type: Indirect immunofluorescence using mAb D3C2 showed that in wild-type embryos, NCL-1 is a cytoplasmic protein with no obvious nuclear accumulation. NCL-1 staining is abundant in early embryos and then rapidly diminishes during development. No staining was detected with mAb D3C2 in ncl-1 (e1942) mutant embryos at any stage. | |||
| In the hermaphrodite gonad: Nucleoli are visible in the syncytial gonad of adult hermaphrodites but disappear as oocytes mature. Cytoplasmic NCL-1 staining is absent in the mitotic region of the syncytial gonad, low in the meiotic region, and then increases throughout oocyte maturation such that the most intense staining is seen in the most mature oocyte. | |||
| Type | Antibody | Mouse monoclonal antibody. | |
| Picture | WBPicture0000008902 | ||
| Remark | No detailed description on cellular expression pattern. | ||
| Reference | WBPaper00003044 | ||
| Antibody_info | WBAntibody00000166 |
