WormBase Tree Display for Expr_pattern: Expr13498
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| Expr13498 | Expression_of | Gene | WBGene00003124 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003124 | |||
| Expression_data | Anatomy_term | WBbt:0003679 | Certain | |
| WBbt:0005733 | Certain | |||
| WBbt:0005751 | Certain | |||
| WBbt:0005755 | Certain | |||
| WBbt:0005773 | Certain | |||
| WBbt:0006748 | Certain | |||
| GO_term | GO:0005634 | |||
| GO:0005737 | ||||
| Type | Reporter_gene | |||
| Pattern | GFP::MAI-1 (xmSi32) and MAI-1::mCherry (xmSi31) showed the same tissue localization. GFP::MAI-1 is expressed in specific somatic tissues in the nuclei and in the cytoplasm through specific tissues, including the cuticle, hypodermis, rectum, vulva and neurons. Although the expression pattern did not suggest a mitochondria network and MAI-1 does not carry a mitochondrial transport sequence, we tested whether MAI-1 was expressed in the mitochondria by incubating GFP:: MAI-1 with Mitotracker Red CMXRos and found no co-localization between the mitochondrial probe and GFP::MAI-1. These results demonstrate that MAI-1 is indeed not expressed in mitochondria but in the cytoplasm, as previously suggested Ichikawa et al. Additionally, we observed that GFP::MAI-1 is expressed in the nuclei. | |||
| Picture | WBPicture0000014626 | |||
| Reference | WBPaper00052941 | |||
| Transgene | WBTransgene00024534 | |||
| WBTransgene00024535 |
