WormBase Tree Display for Expr_pattern: Expr1347
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| Expr1347 | Expression_of | Gene | WBGene00004373 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004373 | |||
| Expression_data | Life_stage | WBls:0000002 | ||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005751 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0005784 | Certain | |||
| GO_term | GO:0005737 | |||
| GO:0012505 | ||||
| Subcellular_localization | Throughout larval and adult stages, anti-RME-1 antibodies detected RME-1 associated with membranes and RME-1 diffuse in the cytoplasm of all cells. In germ cells, RME-1 was strongly concentrated in punctate structures underneath the plasma membrane. RME-1 was also present in the cytoplasm of germ cells, but was not found in nuclei. This was true for immature germ cells and for fully grown oocytes. Cytoplasmic RME-1 in intestinal cells was less diffuse than in germ cells. RME-1 was observed in the cytoplasm of intestinal cells in three different localizations: in small punctate structures (probably vesicles), in tubulo-vesicular structures near the basal (but not apical) plasma membrane, and in rings around the periphery of large vesicular structures, deeper within the cytoplasm. In some preparations, the rings appeared smooth, and were variegated with regions of high and low labelling in other preparations. Further experiments indicated that some of these structures, if not all, are endosomes. RME-1 is a peripheral endosomal protein in the intestine, and is localized in structures arising from basolateral endocytosis. | |||
| Type | Antibody | Affinity-purified rabbit polyclonal anti-RME-1 antibodies raised against recombinant protein. | ||
| Pattern | RME-1 was most abundant in the germline, intestine, pharynx and coelomocytes. | |||
| Reference | WBPaper00004718 | |||
| Antibody_info | WBAntibody00000371 |
