WormBase Tree Display for Expr_pattern: Expr1325
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| Expr1325 | Expression_of | Gene | WBGene00006771 | |
|---|---|---|---|---|
| WBGene00003012 | ||||
| Reflects_endogenous_expression_of | WBGene00003012 | |||
| WBGene00006771 | ||||
| Homol | Homol_homol | CHROMOSOME_II:Expr | ||
| Expression_data | Life_stage | WBls:0000003 | ||
| Anatomy_term | WBbt:0004421 | Certain | ||
| WBbt:0004575 | Certain | |||
| WBbt:0004576 | Certain | |||
| WBbt:0005733 | Certain | |||
| WBbt:0006875 | Certain | |||
| WBbt:0007831 | Certain | |||
| WBbt:0008435 | Certain | |||
| GO_term | GO:0005737 | |||
| GO:0016328 | ||||
| Type | Antibody | LIN-26 antibody | ||
| In_situ | Using antisense probes corresponding to probes used to screen cDNA libraries. | |||
| Reporter_gene | ||||
| Pattern | The GFP::TLN-1 reporter showed prominent expression in all differentiating vulval and uterine cells. In addition to the basal and lateral plasma membrane- associated signal, GFP::TLN-1 expression was also observed in the cytoplasm of the vulval cells of wild-type L3 larvae. | |||
| lin-26 transcripts were first weakly detected by in situ hybridization in the germline precursors (P3, P4 and Z2/Z3) until the 50-cell stage and occasionally in their sisters. After the 100-cell stage, lin-26 transcripts and the beta-galactosidase encoded by the lin-26::lacZ construct were detected in hypodermal precursors, then in differentiating hypodermal and support cells. The only difference between in situ hybridization and antibody staining is that lin-26 transcripts could not be detected in body hypodermal cells after the 350-cell stage nor in any cell after the comma stage, whereas LIN-26 protein can be detected until the end of embryogenesis in all hypodermal cells and in all support cells. | ||||
| Picture | WBPicture0000011694 | |||
| WBPicture0000011695 | ||||
| Remark | Reporter gene fusion type not specified. | |||
| Reference | WBPaper00050698 | |||
| WBPaper00003532 | ||||
| Transgene | WBTransgene00027463 | |||
| WBTransgene00032083 | ||||
| Antibody_info | WBAntibody00000122 |
