WormBase Tree Display for Expr_pattern: Expr13175
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| Expr13175 | Expression_of | Gene | WBGene00022106 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00022106 | |||
| Expression_data | Anatomy_term | WBbt:0005190 | Certain | |
| WBbt:0005735 | Certain | |||
| WBbt:0006840 | Certain | |||
| GO_term | GO:0098793 | |||
| Subcellular_localization | In the DA9 cholinergic motor neuron, which synapses onto dorsal body wall muscles, LGC-46::GFP was detected only in the dorsal side of L4 and adult animals. These results indicate that LGC-46 primarily localizes to the axonal compartment of cholinergic motor neurons. To determine if the punctate pattern of LGC-46::GFP corresponded to presynaptic terminals, we co-immunostained LGC-46::GFP together with endogenous active zone protein RIM/UNC-10. LGC-46::GFP displayed a high degree of overlap with UNC-10. Quantitative analyses of colocalization indicate that LGC-46 localizes to the release sites of synaptic vesicles (SVs). Additionally, LGC-46::GFP showed co-localization with a synaptic vesicle marker mCherry::RAB-3 expressed in cholinergic motor neurons. To our knowledge LGC-46 is the first reported LGIC localized to presynaptic terminals in C. elegans. | |||
| Type | Reporter_gene | |||
| Pattern | lgc-46 is broadly expressed in the nervous system, including motor neurons. Co-expression of Plgc-46-GFP with markers for cholinergic (Punc-17-mCherry) and GABAergic (Pttr-39-mCherry) neurons revealed that lgc-46 is strongly expressed in cholinergic motor neurons, and weakly expressed in GABAergic motor neurons. | |||
| Reference | WBPaper00050365 | |||
| Transgene | WBTransgene00023959 | |||
| WBTransgene00023960 | ||||
| WBTransgene00023961 |
