WormBase Tree Display for Expr_pattern: Expr1290
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| Expr1290 | Expression_of | Gene | WBGene00000098 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000098 | ||||
| Expression_data | Life_stage | WBls:0000003 | |||
| WBls:0000041 | |||||
| Anatomy_term | WBbt:0004506 | Certain | |||
| WBbt:0004520 | Certain | ||||
| WBbt:0005784 | Partial | ||||
| Remark | Expressed in mitotic germ cells. | ||||
| GO_term | GO:0005737 | ||||
| GO:0005813 | |||||
| Subcellular_localization | Diffuse cytoplasmic staining was found during interphase but, as cells entered mitosis, AIR-1 became localized to the centrosomes and remained associated with them throughout the mitotic phase of the cell cycle. Developmentally, AIR-1 was diffuse throughout oocytes and was not detected on the centrosomes of the sperm pronucleus. AIR-1 staining of centrosomes was initially detected in the first mitotic division following pronuclear fusion, and was present in all mitotic cells up to the limits of detection in the hundreds of cells comprising late-stage embryos. | ||||
| Type | Antibody | Rabbit polyclonal antiserum was raised against a synthetic peptide corresponding to the carboxyl-terminal 12 amino acids of AIR-1. | |||
| Pattern | Immunocytochemistry using affinity-purified antibodies on fixed C. elegans embryos of varying ages revealed that AIR-1 is present on mitotic centrosomes. In the adult, AIR-1 staining was also found on the centrosomes of mitotic germ cells in the distal gonad. | ||||
| Picture | WBPicture0000007998 | ||||
| Reference | WBPaper00003325 | ||||
| Antibody_info | WBAntibody00000199 |
