WormBase Tree Display for Expr_pattern: Expr12038

Expr12038 Expression_of: Gene: WBGene00000238
    Reflects_endogenous_expression_of: WBGene00000238
  Expression_data: Life_stage: WBls:0000027 Anatomy_term: WBbt:0006899
    Anatomy_term: WBbt:0006899 Certain
        Life_stage: WBls:0000027
    GO_term: GO:0005737
  Type: Reporter_gene
  Pattern: BAR-1-GFP expression first appeared in P11.p in the late L1 stage. In the early-to-middle L2 stage, BAR-1-GFP accumulated in the cytoplasm of P11.p in a punctate pattern, presumably resulting from the stabilization of BAR-1 in response to increased Wnt signaling. The punctate GFP fluorescence in the cytoplasm of P11. p rapidly decreased during the mid-to-late L2 stage. By the mid-L3 stage, just before P11.p divides, BAR-1-GFP expression appeared to be brighter in the nucleus than in the cytoplasm. The switch of cytoplasmic-to-nuclear BAR-1-GFP accumulation is initiated in the mid-to-late L2 stage, coincident with the time window critical for the specification of HCG cell fates. BAR-1-GFP expression was undetectable in P10.p prior to cell division but became visible in the nucleus of the posterior daughter, P10.pp, suggesting that Wnt signaling through BAR-1 likely acts during fate execution of some descendants of the P10.p lineage. Although we did not observe lin-17::GFP expression in P9.p, faint, mostly cytoplasmic expression of BAR-1-GFP was sometimes seen in P9.p up to the mid-L2 stage, just before P9.p fuses with hyp7.
  Reference: WBPaper00045644
  Transgene: WBTransgene00000528