WormBase Tree Display for Expr_pattern: Expr1199

Expr1199 Expression_of: Gene: WBGene00001177
    Reflects_endogenous_expression_of: WBGene00001177
  Expression_data: Anatomy_term: WBbt:0003679 Certain
      WBbt:0005772 Partial
        Remark: Expressed in posterior intestinal cells.
    GO_term: GO:0043005
      GO:0043025
  Subcellular_localization: Within neurons, EGL-8 stained in both cell somas and in processes.
  Type: Antibody: Polyclonal antibody against the C-terminal region of EGL-8 and used it to visualize EGL-8 protein.
  Pattern: In wild-type worms, EGL-8 staining primarily in the nervous system and the intestine. EGL-8 staining observed in most or all neurons, although the amount of staining varied between individual neurons. In general, neurons in the head and tail ganglia showed stronger staining than the motor neurons of the ventral cord. In the head ganglia, one pair of amphid neurons showed especially strong staining. Staining was not detected in the sublateral processes or in the dorsal cord. EGL-8's intestinal staining was restricted to two lines of staining running down the long axis of the body and to pairs of loop-like structures at regular intervals. The strongest staining was in the posterior region of the intestine. Confocal microscopy determined that the two lines of staining are on the luminal (apical) side of the intestinal cells.
  Picture: WBPicture0000009590
  Remark: The absence of this staining in egl-8 (md1971) and egl-8 (n488) demonstrates its specificity. The lines of EGL-8 staining colocalize with the staining of MH27, a monoclonal antibody that stains epithelial and intestinal adherens junctions. MH27 staining also extended part of the way around each of the loops of EGL-8 staining, which may represent sites where the ends of the intestinal cells join together.
  Reference: WBPaper00003738
  Antibody_info: WBAntibody00000244