WormBase Tree Display for Expr_pattern: Expr11575
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| Expr11575 | Expression_of | Gene | WBGene00006406 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006406 | |||
| Expression_data | Life_stage | WBls:0000002 | ||
| Anatomy_term | WBbt:0003679 | Certain | ||
| WBbt:0003681 | Certain | |||
| WBbt:0005319 | Certain | |||
| WBbt:0005733 | Certain | |||
| WBbt:0006751 | Certain | |||
| WBbt:0006759 | Certain | |||
| WBbt:0008378 | Certain | |||
| GO_term | GO:0005911 | |||
| GO:0043296 | ||||
| Subcellular_localization | A translational fusion protein had the following subcellular localization. In embryos, SRGP-1::GFP localized specifically to sites of contact between cells. When first detected, approximately 150 min after first cleavage, SRGP-1::GFP appeared along most cell-cell contacts. After epidermal differentiation, SRGP-1 appeared uniformly along all apical junctions, where it colocalized with cadherin complex components. SRGP-1 did not colocalize appreciably with DLG-1/discs large and AJM-1, but appeared to reside predominantly apical to the DLG-1-AJM-1 complex. Immunostaining of endogenous SRGP-1 with a polyclonal antibody validated the observations made with the GFP-tagged protein. | |||
| Type | Antibody | Primary antibodies used were rabbit anti-SRGP-1, raised against the last 300 aa of the protein (ProteinTech Group). | ||
| Reporter_gene | ||||
| Pattern | Transcription was detected as early as 100 min into development in all cells except the germline. Later in embryogenesis, expression was restricted to neurons, epidermal cells, and cells of the pharynx. In adults, expression was found in head and tail neurons and in neurons along the body, in addition to strong expression in the pharynx and spermatheca. | |||
| Picture | WBPicture0000013399 | |||
| WBPicture0000013400 | ||||
| Remark | Temporal description | |||
| Reference | WBPaper00037766 | |||
| Transgene | WBTransgene00002330 | |||
| WBTransgene00008311 |
