WormBase Tree Display for Expr_pattern: Expr11270
expand all nodes | collapse all nodes | view schema
| Expr11270 | Expression_of | Gene | WBGene00004681 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004681 | |||
| Expression_data | Anatomy_term | WBbt:0005772 | Certain | |
| GO_term | GO:0005730 | |||
| GO:0005634 | ||||
| GO:0005737 | ||||
| GO:0005783 | ||||
| Subcellular_localization | A reticular pattern was observed for both RSD-2A and RSD-2B isoforms in intestinal cells; however, the smaller RSD-2B protein displayed an additional, more diffuse pattern of staining in the cytoplasm. In double-labeling experiments using anti-GFP and anticalreticulin antibodies, we observed colocalization of fluorescent signals in some animals(50-80%). Calreticulin harbors a KDEL localization motif and resides in the lumen of the endoplasmic reticulum. The localization pattern of GFP in wild type was reticular and similar to the subcellular pattern obtained using fixed tissues. | |||
| While the cytoplasmic compartment contained most of the RSD-2TGFP protein, additional staining for bothRSD-2A and RSD-2B was observed in a subnuclear compartment. We had previously observed nucleolar staining in germline nuclei, oocytes, fertilized embryos, and intestinal cells in fixed animals using anti-GFP antibodies; however, the staining intensity was often weak and was not observed in all immunolocalization experiments. The integrated transgene expressing the RSD-2ATGFP reporter also accumulated GFP fluorescence in the nuclei of live animals. | ||||
| Type | Reporter_gene | |||
| Picture | WBPicture0000013482 | |||
| Reference | WBPaper00044261 | |||
| Transgene | WBTransgene00019182 |
