WormBase Tree Display for Expr_pattern: Expr1089
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Expr1089 | Expression_of | Gene | WBGene00003878 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00003878 | ||
Expression_data | Life_stage | WBls:0000022 | |
Anatomy_term (4) | |||
GO_term | GO:0043005 | ||
GO:0043025 | |||
Subcellular_localization | A fluorescence microscopy image with a standard fluorescein isothiocyanate filter showed AVAR and AVAL neuron cell bodies and the processes. | ||
Type | Reporter_gene | ||
Pattern | According to the paper cgc3971: GFP was expressed exclusively in the interneuron AVA that is involved in the backward locomotion in C. elegans. The relative location of the GFP-positive neuron with that of the surrounding amphid neurons (ASK, ADL, ASI, ASH, and ASJ) establishes the identity of the GFP-expressing neuron as AVA. The GFP expression in these neurons was detected as early as L1 larva stage and persisted into the adult stage. | ||
Also according to the same pers. comm.: ... the Rongo lab has tried to use the promoter fragment for opt-3 to drive expression in AVE specifically. In their hands, the opt-3 promoter is not as specific as we had observed. The Rongo lab see expression in AVEs and also the pharynx and in the touch cells... | |||
But according to personal communication from Michael Krause on Sept 9, 2003 with WormBase curator: ... Turns out we were not correct, expression of this particular transgene was really in the neuron next door, AVE L&R. That information has been confirmed by David Miller and one other person and the transgene is used to show AVEs in WormAtlas... | |||
Picture | WBPicture0000008144 | ||
Reference | WBPaper00003971 | ||
Transgene | WBTransgene00027338 |