WormBase Tree Display for Expr_pattern: Expr1069
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Expr1069 | Expression_of | Gene | WBGene00003052 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00003052 | ||
Expression_data | Life_stage | WBls:0000003 | |
GO_term | GO:0000922 | ||
GO:0005876 | |||
Subcellular_localization | In both early and later embryos, lamins maintained a nearly complete rim-staining pattern during metaphase and early anaphase. The exception to rim staining was near the spindle poles, where Ce-lamin staining became progressively weaker starting in prometaphase, with a large gap at both poles during early anaphase. This local disruption of lamina integrity was consistent with mechanical puncturing by spindle microtubules, as seen in other organisms. Elsewhere, the lamina remained apparently intact through early anaphase and was removed only during mid-late anaphase. In later embryos (30 cells), the lamina appeared to disassemble more extensively at earlier stages (e.g., prometaphase), as deduced from higher levels of cytoplasmic staining at earlier stages of mitosis. The intensity of lamin antibody staining during mitosis was always higher than in interphase. | ||
Type | Antibody | Affinity-purified polyclonal antibodies against Ce-lamin. | |
Picture | WBPicture0000008669 | ||
WBPicture0000008671 | |||
Remark | Ce-lamin(author) = lam-1 (wjc). The specificity of antibody staining for Ce-lamin was confirmed by the lack of signal in preimmune controls and by the 40-fold loss of the rim stain signal in nematodes disrupted for Ce-lamin expression). No detailed description on cellular localization. | ||
Reference | WBPaper00004339 | ||
Antibody_info | WBAntibody00000312 |