WormBase Tree Display for Expr_pattern: Expr10551
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| Expr10551 | Expression_of | Gene | WBGene00000913 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000913 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| Anatomy_term | WBbt:0005829 | Certain | ||
| WBbt:0006748 | Certain | |||
| WBbt:0006760 | Certain | |||
| WBbt:0006865 | Certain | |||
| WBbt:0007809 | Certain | |||
| GO_term | GO:0005634 | |||
| GO:0005886 | ||||
| GO:0005737 | ||||
| Subcellular_localization | The sub-cellular localization of DAF-18::GFP changed over the course of vulval development. In the VPCs of L2 larvae prior to and during induction (Pn.p stage), DAF-18::GFP was predominantly localized in the cytoplasm and the nucleus. However, at the subsequent stages (Pn.px to Pn.pxx stages), DAF- 18::GFP became increasingly localized to the plasma membrane of the vulval cells. Plasma membrane staining peaked at the 'Christmas tree' (Pn.pxxx) stage, when almost all the protein appeared to be localized to the membranes and nuclear staining was reduced to very low levels. | |||
| Type | Reporter_gene | |||
| Pattern | DAF-18::GFP expression was observed in many tissues during all larval stages, including the developing vulva, the uterus, the ventral nerve cord and the distal tip cells. In particular, equal levels of DAF-18::GFP expression were detected in the six VPCs of L2 larvae, and expression persisted in the descendants of the induced VPCs until the Pn.pxxx stage. Besides the vulval cells, the DAF-18::GFP reporter was also expressed at the L3 to L4 larval stages in several cells of the uterus, which is part of the somatic gonad. | |||
| Picture | WBPicture0000012814 | |||
| Reference | WBPaper00041458 | |||
| Transgene | WBTransgene00015975 |
