WormBase Tree Display for Variation: WBVar02157236

WBVar02157236 Name: Public_name: rae297
  Origin: Species: Caenorhabditis elegans
    Status: Live
  Possibly_affects: WBGene00019633 Paper_evidence: WBPaper00062501
      Remark: CGC_name moc-6
  Description: Phenotype: WBPhenotype:0002195 Paper_evidence: WBPaper00062501
        Curator_confirmed: WBPerson712
        Remark: To determine if K10D2.7 is necessary for endogenous Mocosynthesis, we cultured wild-type, rae295, rae296, and rae297 mutant animals on wild-type and (del)moaA E. coli, a mutantbacterial strain that is unable to synthesize Moco. rae295, rae296, and rae297 mutant animals grew well on wild-type (Mocoproducing) E. coli but displayed a completely penetrant developmental arrest when cultured on (del)moaA (Moco deficient) mutant E. coli (Fig. 1C).; Moco deficiency in C. elegans also results in embryonic lethality when maternal animals are deprived of both endogenous andexogenous sources of Moco during reproductive adulthood (Warnhoff and Ruvkun 2019). moc-6 mutant C. elegans motherswere fed wild-type E. coli until the L4 stage of development, one stage prior to reproductive adulthood. These moc-6 mutantL4 mothers were then shifted onto wild-type or moaA mutant E. coli and the hatching rate of their progeny was determined.When moc-6 mutant animals were shifted to a Moco deficient diet, 0% of their embryos hatched (Fig. 1E). In contrast, 100%of moc-6 mutant embryos hatched when their mothers were fed wild-type E. coli. Hatching rates of wild-type embryos werenot affected by the maternal diet (Fig. 1E). Paper_evidence: WBPaper00062501
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00007965 Paper_evidence: WBPaper00062501
              Curator_confirmed: WBPerson712
  Reference: WBPaper00062501
  Remark: [2022-02-17T18:12:08.356Z WBPerson712] New Variation: newly generated moc-6 deletion; WBPaper00062501. 10.17912/micropub.biology.000531 Curator_confirmed: WBPerson712