Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar02125777

expand all nodes | collapse all nodes | view schema

Name Class

WBVar02125777EvidencePaper_evidenceWBPaper00041219
NamePublic_namevt29
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryBY
StatusLive
AffectsInteractorWBInteraction000521894
GeneticsMapX
DescriptionPhenotypeWBPhenotype:0000010Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkThe application of exogenous DA to worms on solid substrate induces motor slowing and paralysis (Schafer and Kenyon 1995). vt29 displayed sensitivity to exogenous DA like that of dat-1 mutants.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004583Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0000179Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
RemarkDopamine neuron degeneration observed in swip-10(vt29).Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
Phenotype_assayGenotypevtIs7 [Pdat-1::gfp]Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
WBPhenotype:0000234Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkDopamine levels are reduced compared with control animals.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004583Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0000273Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkMutants recovered in the screen demonstrated significantly reduced maximal thrashing frequencies in water.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0000905Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
RemarkShrunken or missing CEP cell soma.Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
Phenotype_assayGenotypevtIs7 [Pdat-1::gfp]Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
WBPhenotype:0001236Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
RemarkAnimals have a significant increase in fluorescence of a basal whole body oxidative stress reporter, dvIs19[pgst-4:GFP].Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
Phenotype_assayGenotypedvIs19[pgst-4:GFP]Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
WBPhenotype:0001398Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
RemarkNeurite truncations and breaks in GFP along CEP dendrites.Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
Phenotype_assayGenotypevtIs7 [Pdat-1::gfp]Paper_evidenceWBPaper00053894
Curator_confirmedWBPerson557
WBPhenotype:0002114Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkThis mutant demonstrates a stable Swip phenotype and can be rescued by reserpine pretreatment. Phenotype not lost after 3X outcrossing. vt29 displayed fully penetrant Swip, yielding ~100% paralysis in manual scoring assays, and an even more rapid rate of paralysis than dat-1. vt29 differed from other mutant lines with a significantly reduced latency to paralyze. vt29 demonstrated a significantly reduced reversion incidence from other lines (and dat-1), which can be readily detected in both the heat map plots and in randomly selected individual thrashing traces. GFP:DAT-1 overexpression only modestly suppressed vt29.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
PenetranceComplete100%Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00002955Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0002233Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkAnimals exhibited a virtually identical sensitivity to osmolarity as dat-1, except a slight, yet significantly increased paralysis at greater osmolarities. In M9, vt29 animals exhibited a significantly greater latency to paralyze than dat-1 animals, opposite to their relative sensitivities in water. Both dat-1 and vt29 lines demonstrated an increase in the number of revertants in M9 as compared with water without changing the length of reversion events.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0002375Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
Remarkvt29 demonstrated a significantly reduced reversion incidence from other lines (and dat-1), which can be readily detected in both heat map plots and in randomly selected individual thrashing traces.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000905Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkCEP, ADE and PDE cell bodies and processes appear unaltered relative to N2 animals.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005244PATO:0000460Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBbt:0005415PATO:0000460Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBbt:0006747PATO:0000460Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBbt:0005278PATO:0000460Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0000961Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkWe could detect no changes in the pattern of GFP::DAT-1 localization, comparing animals with GFP::DAT-1 expressed on an otherwise wild-type background.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0001502Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
Remarkvt29 demonstrated full sensitivity to the toxin, 6-OHDAPaper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00005087Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005278PATO:0000460Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
ReferenceWBPaper00041219
WBPaper00053894
MethodAllele