WormBase Tree Display for Variation: WBVar02125777
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WBVar02125777 | Evidence | Paper_evidence | WBPaper00041219 | ||||||
---|---|---|---|---|---|---|---|---|---|
Name | Public_name | vt29 | |||||||
Sequence_details | SeqStatus | Pending_curation | |||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Laboratory | BY | ||||||||
Status | Live | ||||||||
Affects | Interactor | WBInteraction000521894 | |||||||
Genetics | Map | X | |||||||
Description | Phenotype | WBPhenotype:0000010 | Paper_evidence | WBPaper00041219 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | The application of exogenous DA to worms on solid substrate induces motor slowing and paralysis (Schafer and Kenyon 1995). vt29 displayed sensitivity to exogenous DA like that of dat-1 mutants. | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00004583 | Paper_evidence | WBPaper00041219 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000179 | Paper_evidence | WBPaper00053894 | |||||||
Curator_confirmed | WBPerson557 | ||||||||
Remark | Dopamine neuron degeneration observed in swip-10(vt29). | Paper_evidence | WBPaper00053894 | ||||||
Curator_confirmed | WBPerson557 | ||||||||
Phenotype_assay | Genotype | vtIs7 [Pdat-1::gfp] | Paper_evidence | WBPaper00053894 | |||||
Curator_confirmed | WBPerson557 | ||||||||
WBPhenotype:0000234 | Paper_evidence | WBPaper00041219 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Dopamine levels are reduced compared with control animals. | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00004583 | Paper_evidence | WBPaper00041219 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000273 | Paper_evidence | WBPaper00041219 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Mutants recovered in the screen demonstrated significantly reduced maximal thrashing frequencies in water. | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000905 | Paper_evidence | WBPaper00053894 | |||||||
Curator_confirmed | WBPerson557 | ||||||||
Remark | Shrunken or missing CEP cell soma. | Paper_evidence | WBPaper00053894 | ||||||
Curator_confirmed | WBPerson557 | ||||||||
Phenotype_assay | Genotype | vtIs7 [Pdat-1::gfp] | Paper_evidence | WBPaper00053894 | |||||
Curator_confirmed | WBPerson557 | ||||||||
WBPhenotype:0001236 | Paper_evidence | WBPaper00053894 | |||||||
Curator_confirmed | WBPerson557 | ||||||||
Remark | Animals have a significant increase in fluorescence of a basal whole body oxidative stress reporter, dvIs19[pgst-4:GFP]. | Paper_evidence | WBPaper00053894 | ||||||
Curator_confirmed | WBPerson557 | ||||||||
Phenotype_assay | Genotype | dvIs19[pgst-4:GFP] | Paper_evidence | WBPaper00053894 | |||||
Curator_confirmed | WBPerson557 | ||||||||
WBPhenotype:0001398 | Paper_evidence | WBPaper00053894 | |||||||
Curator_confirmed | WBPerson557 | ||||||||
Remark | Neurite truncations and breaks in GFP along CEP dendrites. | Paper_evidence | WBPaper00053894 | ||||||
Curator_confirmed | WBPerson557 | ||||||||
Phenotype_assay | Genotype | vtIs7 [Pdat-1::gfp] | Paper_evidence | WBPaper00053894 | |||||
Curator_confirmed | WBPerson557 | ||||||||
WBPhenotype:0002114 | Paper_evidence | WBPaper00041219 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | This mutant demonstrates a stable Swip phenotype and can be rescued by reserpine pretreatment. Phenotype not lost after 3X outcrossing. vt29 displayed fully penetrant Swip, yielding ~100% paralysis in manual scoring assays, and an even more rapid rate of paralysis than dat-1. vt29 differed from other mutant lines with a significantly reduced latency to paralyze. vt29 demonstrated a significantly reduced reversion incidence from other lines (and dat-1), which can be readily detected in both the heat map plots and in randomly selected individual thrashing traces. GFP:DAT-1 overexpression only modestly suppressed vt29. | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Penetrance | Complete | 100% | Paper_evidence | WBPaper00041219 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00002955 | Paper_evidence | WBPaper00041219 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002233 | Paper_evidence | WBPaper00041219 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals exhibited a virtually identical sensitivity to osmolarity as dat-1, except a slight, yet significantly increased paralysis at greater osmolarities. In M9, vt29 animals exhibited a significantly greater latency to paralyze than dat-1 animals, opposite to their relative sensitivities in water. Both dat-1 and vt29 lines demonstrated an increase in the number of revertants in M9 as compared with water without changing the length of reversion events. | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002375 | Paper_evidence | WBPaper00041219 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | vt29 demonstrated a significantly reduced reversion incidence from other lines (and dat-1), which can be readily detected in both heat map plots and in randomly selected individual thrashing traces. | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_not_observed | WBPhenotype:0000905 | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | CEP, ADE and PDE cell bodies and processes appear unaltered relative to N2 animals. | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005244 | PATO:0000460 | Paper_evidence | WBPaper00041219 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBbt:0005415 | PATO:0000460 | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBbt:0006747 | PATO:0000460 | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBbt:0005278 | PATO:0000460 | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000961 | Paper_evidence | WBPaper00041219 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | We could detect no changes in the pattern of GFP::DAT-1 localization, comparing animals with GFP::DAT-1 expressed on an otherwise wild-type background. | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001502 | Paper_evidence | WBPaper00041219 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | vt29 demonstrated full sensitivity to the toxin, 6-OHDA | Paper_evidence | WBPaper00041219 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00005087 | Paper_evidence | WBPaper00041219 | |||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005278 | PATO:0000460 | Paper_evidence | WBPaper00041219 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Reference | WBPaper00041219 | ||||||||
WBPaper00053894 | |||||||||
Method | Allele |