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WormBase Tree Display for Variation: WBVar00531947

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Name Class

WBVar00531947EvidencePaper_evidenceWBPaper00036384
NamePublic_namebp412
Sequence_detailsMapping_targetY55F3AM
SeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00008597
LaboratoryHZ
StatusLive
AffectsGeneWBGene00021922
InteractorWBInteraction000520089
WBInteraction000525193
WBInteraction000525218
WBInteraction000525234
WBInteraction000525236
WBInteraction000525237
WBInteraction000525238
WBInteraction000525244
WBInteraction000525248
WBInteraction000525287
DescriptionPhenotypeWBPhenotype:0000067Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
RemarkA few ALG-1 and ALG-2 aggregates were formed in autophagy mutants but the number of aggregates dramatically increased under certain stress conditions, unlike in Wild type controls.Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
WBPhenotype:0000119Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
RemarkLevels of endogenous AIN-1 protein, but not ain-1 mRNA, were increased in autophagy mutants.Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
WBPhenotype:0000243Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
RemarkThe atg-3(bp412) mutation resulted in a significant increase in the duration time of cell corpses observed in embryos (Figure S1D)Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
In the piki-1(ok2346) mutant background, the atg-3(bp412) mutation resulted in a significant increase in the duration time of cell corpses observed in embryos, compared to piki-1(ok2346) controls (Figure S1D)Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Phenotype_assayGenotypepiki-1(ok2346)Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
WBPhenotype:0000679Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
RemarkAuthors found that phagosomal association of GFP-RAB-5, which is recruited at early phagosome maturation stages, decreased significantly in atg-3(bp412) mutants (Figure 1D, G).Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Authors found that phagosomal association of GFP-RAB-7, which is recruited at late phagosome maturation stages, decreased significantly in atg-3(bp412) mutants (Figure 1E, H).Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Phagosomal labeling by the lysosomal membrane protein LAAT-1 was reduced in atg-3(bp412) mutants (Figure 1F, I).Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeGFP-RAB-5Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
GFP-RAB-7Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
LAAT-1-mCHERRYPaper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
WBPhenotype:0000885Paper_evidenceWBPaper00041694
Curator_confirmedWBPerson2798
Remarkdelayed engulfment of apoptotic corpses during embryogenesisPaper_evidenceWBPaper00041694
Curator_confirmedWBPerson2798
WBPhenotype:0000961Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
RemarkIn wild-type embryos, AIN-1::GFP was diffusely expressed in the cytoplasm during embryogenesis. In autophagy mutants AIN-1::GFP was strongly expressed and accumulated into a large number of aggregates. Expression of AIN-2::GFP, which is diffusely localized in wild-type embryos, was unaffected in autophagy mutant embryos. Translational fusion reporters for gfp::alg-1 and alg-2::gfp were both diffusely expressed in the cytoplasm of most embryonic cells.Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
WBPhenotype:0001181Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
RemarkThe atg-3(bp412) mutation resulted in a significant increase in the number of cell corpses observed in embryos (Table 1, Figure S1A,B)Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
WBPhenotype:0001405Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
RemarkAIN-1::GFP aggregates largely colocalized with SQST-1 aggregates. AIN-1 colocalizes with known protein aggregates in autophagy mutants. GFP::ALG-1 and ALG-2::GFP aggregates also colocalized with SQST-1 aggregates but were separable from PGL granules in atg-3 mutants.Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
WBPhenotype:0001846Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
RemarkIn atg-3(bp412) mutants, authors observed that the CED-1-GFP ring formed normally but persisted a little longer on phagosomes than in wild type, suggesting possible defects in phagosome maturation (Figure 1C).Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Authors found that phagosomal association of GFP-RAB-5, which is recruited at early phagosome maturation stages, decreased significantly in atg-3(bp412) mutants (Figure 1D, G).Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Authors found that phagosomal association of GFP-RAB-7, which is recruited at late phagosome maturation stages, decreased significantly in atg-3(bp412) mutants (Figure 1E, H).Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Phagosomal labeling by the lysosomal membrane protein LAAT-1 was reduced in atg-3(bp412) mutants (Figure 1F, I).Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
atg-3(bp412) mutants exhibited no significant delay in the recruitment of phosphatidylinositol 3-phosphate (PI3P) to corpse-engulfing phagosomes, as determined by the PI3P marker YFP-2xFYVE (Figure 3B,H, S3C,D), but the duration of the phagosomal YFP-2xFYVE signal was shorter than in wild type (Figure 3B and G-I).Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeCED-1-GFPPaper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
GFP-RAB-5Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
GFP-RAB-7Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
LAAT-1-mCHERRYPaper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
YFP-2xFYVE, a marker for phosphatidylinositol 3-phosphatePaper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
WBPhenotype:0002349Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
RemarkAuthors found significantly reduced YFP-2xFYVE labeling of cell corpses in atg-3(bp412) mutants, suggesting that phosphatidylinositol 3-phosphate (PtdIns3P) generation and/or accumulation on phagosomes is affected (Figure 2A, B).Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeYFP-2xFYVE, a marker for phosphatidylinositol 3-phosphatePaper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000114Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
RemarkMutants exhibited normal alg-1 and alg-2 and mRNA levels.Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
WBPhenotype:0000590Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Remarkatg-3(bp412) mutants did not exhibit changes in the number of germ cell corpses that were observed in animals 48 hours post-L4 larval stage (Figure S1E)Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
WBPhenotype:0000701Paper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
Remarkmutants exhibited normal seam cell developmentPaper_evidenceWBPaper00042320
Curator_confirmedWBPerson712
WBPhenotype:0000885Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Remarkatg-3(bp412) mutants exhibited wild type kinetics of CED-1::GFP ring-like structure formation around cell corpses (Figure 1A,B)Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeCED-1-GFPPaper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
WBPhenotype:0001301Paper_evidenceWBPaper00036384
Curator_confirmedWBPerson712
RemarkP granules were spherical and evenly dispersed. Localization patterns were distinct from the localization of T12G3.1 fluorescent signals.Paper_evidenceWBPaper00036384
Curator_confirmedWBPerson712
WBPhenotype:0001346Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
RemarkActin cytoskeleton changes (assembly and disassembly) during cell corpse engulfment in atg-3(bp412) mutants were the same as in wild type (Fig. S2A and S2B)Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Actin ring formation on corpse-engulfing phagosomes was not affected by the atg-3(bp412) mutation (Figure S3A,B), in the piki-1(ok2346) mutant backgroundPaper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeGFP-ACT-1Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
piki-1(ok2346); GFP-ACT-1Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
WBPhenotype:0001846Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
RemarkActin ring formation on corpse-engulfing phagosomes was not affected by the atg-3(bp412) mutation (Figure S3A,B), in the piki-1(ok2346) mutant backgroundPaper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
Phenotype_assayGenotypepiki-1(ok2346); GFP-ACT-1Paper_evidenceWBPaper00044390
Curator_confirmedWBPerson2987
ReferenceWBPaper00041694
WBPaper00036384
WBPaper00042320
WBPaper00044390
WBPaper00065267
WBPaper00065712
MethodAllele