WormBase Tree Display for Variation: WBVar00275520

WBVar00275520 Name: Public_name: zu193
  Sequence_details: SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00007281
      WBStrain00007282
    Laboratory: JJ
    Status: Live
  Affects: Gene: WBGene00003397
    Interactor (14)
  Description: Phenotype: WBPhenotype:0000047 Paper_evidence: WBPaper00038341
        Curator_confirmed: WBPerson712
        Remark: Cells internalize late at the 4E stage in 72% of embryos. Paper_evidence: WBPaper00038341
            Curator_confirmed: WBPerson712
      WBPhenotype:0000050 Paper_evidence: WBPaper00002871
        Curator_confirmed: WBPerson2987
        Penetrance: Complete: Paper_evidence: WBPaper00002871
              Curator_confirmed: WBPerson2987
      WBPhenotype:0000219 Paper_evidence: WBPaper00031110
        Curator_confirmed: WBPerson712
        Remark: Animals from strains from either the Sternberg lab or the Eisenmann lab exhibit an underinduction phenotype. Paper_evidence: WBPaper00031110
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00031110
            Curator_confirmed: WBPerson712
      WBPhenotype:0000436 Paper_evidence: WBPaper00026736
        Curator_confirmed: WBPerson2021
        Remark: WRM-1 fails to be retained in the E nucleus Paper_evidence: WBPaper00026736
            Curator_confirmed: WBPerson2021
        EQ_annotations (2)
        Phenotype_assay: Treatment: mom-5 mutants carrying the WRM-1::GFP transgene were examined. Nuclear retention was assayed by subjecting the animals to imb-4 RNAi Paper_evidence: WBPaper00026736
              Curator_confirmed: WBPerson2021
          Genotype: neIs2 [WRM-1::GFP] Paper_evidence: WBPaper00026736
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000472 Paper_evidence: WBPaper00002871
        Curator_confirmed: WBPerson2987
        Remark: Table 1 Paper_evidence: WBPaper00002871
            Curator_confirmed: WBPerson2987
        Penetrance: Low: 5% penetrance Paper_evidence: WBPaper00002871
              Curator_confirmed: WBPerson2987
      WBPhenotype:0000760 Paper_evidence: WBPaper00003645
        Curator_confirmed: WBPerson2987
        Remark: In mom-5(zu193) mutants, the EMS cell exhibited spindle orientation defects, with significant dorsal/ventral or left/right orientation components, in contrast to the normally anterior/posterior orientation of the wild type EMS spindle (data not shown). Paper_evidence: WBPaper00003645
            Curator_confirmed: WBPerson2987
          In EMS cells derived from isolated P1 blastomeres from mom-5(zu193) mutants, spindles exhibited orientation defects, with a wide range of orientation angles, showing nearly random orientations (Figure 2). Paper_evidence: WBPaper00003645
            Curator_confirmed: WBPerson2987
          In EMS blastomeres isolated from mom-5(zu193) mutant embryos and placed in contact with isolated wild type P2 blastomeres, EMS spindles exhibited orientation defects suggesting that mom-5 is required in EMS for proper EMS spindle orientation (Figure 4A). Paper_evidence: WBPaper00003645
            Curator_confirmed: WBPerson2987
        Penetrance: Incomplete: 57% penetrance Paper_evidence: WBPaper00003645
              Curator_confirmed: WBPerson2987
        EQ_annotations (2)
        Phenotype_assay: Treatment: Authors isolated the smaller, posterior-most blastomere in a two-cell-stage embryo, called P1, and allowed it to divide into its daughters, P2 and EMS. The orientation of the mitotic spindle in EMS was then measured relative to the plane of contact between EMS and P2. Paper_evidence: WBPaper00003645
              Curator_confirmed: WBPerson2987
            For genetically mosaic partial embryos, wild-type and mutant early EMS and P2 blastomeres were concurrently isolated. EMS and P2 were recombined early in the EMS cell cycle allowing the cells to be in contact for at least 9 min before EMS cytokinesis. Paper_evidence: WBPaper00003645
              Curator_confirmed: WBPerson2987
      WBPhenotype:0001495 Paper_evidence: WBPaper00002871
        Curator_confirmed: WBPerson2987
        Remark: E daughter cells of some (2/12) mom-5(zu193) mutant embryos divide earlier (compared to MS daughters) than in wild type embryos Paper_evidence: WBPaper00002871
            Curator_confirmed: WBPerson2987
        Penetrance: Incomplete: Paper_evidence: WBPaper00002871
              Curator_confirmed: WBPerson2987
        EQ_annotations: Anatomy_term: WBbt:0006733 PATO:0000460 Paper_evidence: WBPaper00002871
                Curator_confirmed: WBPerson2987
            WBbt:0006612 PATO:0000460 Paper_evidence: WBPaper00002871
                Curator_confirmed: WBPerson2987
      WBPhenotype:0001635 Paper_evidence: WBPaper00002871
        Curator_confirmed: WBPerson2987
        Remark: E blastomeres produced pharyngeal cells (aberrantly) instead of endoderm in 2/15 (13%) mom-5(zu193) mutants Paper_evidence: WBPaper00002871
            Curator_confirmed: WBPerson2987
        EQ_annotations: Anatomy_term: WBbt:0004804 PATO:0000460 Paper_evidence: WBPaper00002871
                Curator_confirmed: WBPerson2987
      WBPhenotype:0001637 Paper_evidence: WBPaper00005375
        Curator_confirmed: WBPerson2987
        Remark: 4% of mom-5(zu193) embryos lacked an intestine (Table 1) Paper_evidence: WBPaper00005375
            Curator_confirmed: WBPerson2987
        Penetrance: Low: 4 Paper_evidence: WBPaper00005375
              Curator_confirmed: WBPerson2987
        EQ_annotations: Anatomy_term: WBbt:0005772 PATO:0000460 Paper_evidence: WBPaper00005375
                Curator_confirmed: WBPerson2987
    Phenotype_not_observed (5)
  Reference: WBPaper00038341
    WBPaper00005375
    WBPaper00002871
    WBPaper00026736
    WBPaper00003645
    WBPaper00031110
    WBPaper00035405
    WBPaper00060654
  Method: Allele