WormBase Tree Display for Variation: WBVar00266710

WBVar00266710 Evidence: Paper_evidence: WBPaper00004813
  Name: Public_name: ua2
    HGVSg: CHROMOSOME_III:g.5227862_5228571del
  Sequence_details: SMap: S_parent: Sequence: C34E10
    Flanking_sequences: ttggcaatcgaggtggcgtagttgagaagc tcagctcaaacaatgttgagtccaagaagg
    Mapping_target: C34E10
    Type_of_mutation: Deletion
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00024104
      WBStrain00024105
    Laboratory: LB
    Status: Live
  Affects: Gene: WBGene00000229
      WBGene00303005
      WBGene00016411
    Transcript: C34E10.12a.1 VEP_consequence: coding_sequence_variant,5_prime_UTR_variant
        VEP_impact: MODIFIER
        cDNA_position: ?-20
        CDS_position: ?-20
        Protein_position: ?-7
        Exon_number: 1/1
      C34E10.6.2 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        cDNA_position: ?-108
        CDS_position: ?-97
        Protein_position: ?-33
        Intron_number: 2/7
        Exon_number: 1-3/8
      C34E10.6.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        cDNA_position: ?-108
        CDS_position: ?-97
        Protein_position: ?-33
        Intron_number: 2/6
        Exon_number: 1-3/7
      C34E10.10.1 VEP_consequence: 5_prime_UTR_variant
        VEP_impact: MODIFIER
        cDNA_position: ?-23
        Exon_number: 1/5
  Isolation: Mutagen: EMS
  Genetics: Interpolated_map_position: III -1.93862
    Mapping_data: In_multi_point: 4167
  Description: Phenotype: WBPhenotype:0000050 Paper_evidence: WBPaper00024944
        Curator_confirmed: WBPerson712
      WBPhenotype:0000083 Paper_evidence: WBPaper00024944
        Curator_confirmed: WBPerson712
      WBPhenotype:0000523 Paper_evidence: WBPaper00058699
        Curator_confirmed: WBPerson712
        Remark: We found that isp-1 and atp-2 strains had decreased worm length when exposed to low concentrations of sodium arsenite. In the 8-day old worms, the nuo-6, mev-1, and atp-2 strains were more sensitive to sodium arsenite compared to the N2 strain. Synchronized worms were exposed to 500 M sodium arsenite beginning 2 days post hatch for 48 hours. Immediately after the exposure, steady state ATP levels were measured using the Promega CellTiterGlo Luminescent assay and normalized to protein per worm (adapted from Bailey et al. 2016). Although we report no significant differences, there is a strong trend of decreased ATP in the nuo-6 and atp-2 mutant strains Paper_evidence: WBPaper00058699
            Curator_confirmed: WBPerson712
        Image: WBPicture0000014932 Paper_evidence: WBPaper00058699
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00001860 Paper_evidence: WBPaper00058699
              Curator_confirmed: WBPerson712
  Reference: WBPaper00024944
    WBPaper00005808
    WBPaper00058699
  Remark: Corrected flanks based on U10402.1 genbank sequence.
  Method: Deletion_allele