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WormBase Tree Display for Variation: WBVar00239433

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Name Class

WBVar00239433EvidencePaper_evidenceWBPaper00031945
NamePublic_namepk2247
Sequence_detailsMapping_targetF33H2
Type_of_mutationSubstitutionct
SeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryNL
StatusLive
AffectsGeneWBGene00001049
DescriptionPhenotypeWBPhenotype:0001760Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
RemarkMonoG or G-tracts were deleted at an increased rate, in all somatic tissues, as demonstrated by the expression of B-galactosidase as an indicator of a DNA rearrangement bringing a LacZ start codon in-frame with the downstream ORF. MonoA tracts were not susceptible to deletions. An increase in deletion frequency over wild-type was observed for the endogenous qua375 sequence as determined by PCR. At least 24 populations of 5 animals were assayed.Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
Phenotype_assayGenotypepkIs2165[pRP1878: hsp-16.41::ATG-(C)23-stops-LacZ unc-119] or pkIs2172 [pRP1889: hsp-16.41::ATG-(monoA)-stops-LacZ unc-119]Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
WBPhenotype:0001781Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
RemarkEmbryonic lethality is increased compared to N2 for animals exposed to crosslinking agent Cisplatin and mutagen di-epoxybutane.Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals of the larval L4 stage were treated with 0-0.2mM di-epoxybutane or 0-0.9mMCisPt, and the ratio live progeny/total laid eggs was determined for a 24 hr time window 24 hr after treatment.Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
GenotypepkIs2165[pRP1878: hsp-16.41::ATG-(C)23-stops-LacZ unc-119] or pkIs2172 [pRP1889: hsp-16.41::ATG-(monoA)-stops-LacZ unc-119]Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000711Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
RemarkEmbryonic lethality is not increased compared to N2 for animals exposed to ionizing radiation.Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals of the larval L4 stage were treated with 0-100 Gy of ionizing radiation, and the ratio live progeny/total laid eggs was determined for a 24 hr time window 24 hr after treatment.Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
GenotypepkIs2165[pRP1878: hsp-16.41::ATG-(C)23-stops-LacZ unc-119] or pkIs2172 [pRP1889: hsp-16.41::ATG-(monoA)-stops-LacZ unc-119]Paper_evidenceWBPaper00031945
Curator_confirmedWBPerson712
ReferenceWBPaper00031945
MethodSubstitution_allele