WormBase Tree Display for Variation: WBVar00239433
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WBVar00239433 | Evidence | Paper_evidence | WBPaper00031945 | |||||
---|---|---|---|---|---|---|---|---|
Name | Public_name | pk2247 | ||||||
Sequence_details | Mapping_target | F33H2 | ||||||
Type_of_mutation | Substitution | c | t | |||||
SeqStatus | Pending_curation | |||||||
Variation_type | Allele | |||||||
Origin | Species | Caenorhabditis elegans | ||||||
Laboratory | NL | |||||||
Status | Live | |||||||
Affects | Gene | WBGene00001049 | ||||||
Description | Phenotype | WBPhenotype:0001760 | Paper_evidence | WBPaper00031945 | ||||
Curator_confirmed | WBPerson712 | |||||||
Remark | MonoG or G-tracts were deleted at an increased rate, in all somatic tissues, as demonstrated by the expression of B-galactosidase as an indicator of a DNA rearrangement bringing a LacZ start codon in-frame with the downstream ORF. MonoA tracts were not susceptible to deletions. An increase in deletion frequency over wild-type was observed for the endogenous qua375 sequence as determined by PCR. At least 24 populations of 5 animals were assayed. | Paper_evidence | WBPaper00031945 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Genotype | pkIs2165[pRP1878: hsp-16.41::ATG-(C)23-stops-LacZ unc-119] or pkIs2172 [pRP1889: hsp-16.41::ATG-(monoA)-stops-LacZ unc-119] | Paper_evidence | WBPaper00031945 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001781 | Paper_evidence | WBPaper00031945 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Embryonic lethality is increased compared to N2 for animals exposed to crosslinking agent Cisplatin and mutagen di-epoxybutane. | Paper_evidence | WBPaper00031945 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | Animals of the larval L4 stage were treated with 0-0.2mM di-epoxybutane or 0-0.9mMCisPt, and the ratio live progeny/total laid eggs was determined for a 24 hr time window 24 hr after treatment. | Paper_evidence | WBPaper00031945 | ||||
Curator_confirmed | WBPerson712 | |||||||
Genotype | pkIs2165[pRP1878: hsp-16.41::ATG-(C)23-stops-LacZ unc-119] or pkIs2172 [pRP1889: hsp-16.41::ATG-(monoA)-stops-LacZ unc-119] | Paper_evidence | WBPaper00031945 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_not_observed | WBPhenotype:0000711 | Paper_evidence | WBPaper00031945 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Embryonic lethality is not increased compared to N2 for animals exposed to ionizing radiation. | Paper_evidence | WBPaper00031945 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | Animals of the larval L4 stage were treated with 0-100 Gy of ionizing radiation, and the ratio live progeny/total laid eggs was determined for a 24 hr time window 24 hr after treatment. | Paper_evidence | WBPaper00031945 | ||||
Curator_confirmed | WBPerson712 | |||||||
Genotype | pkIs2165[pRP1878: hsp-16.41::ATG-(C)23-stops-LacZ unc-119] or pkIs2172 [pRP1889: hsp-16.41::ATG-(monoA)-stops-LacZ unc-119] | Paper_evidence | WBPaper00031945 | |||||
Curator_confirmed | WBPerson712 | |||||||
Reference | WBPaper00031945 | |||||||
Method | Substitution_allele |