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WormBase Tree Display for Variation: WBVar00144578

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Name Class

WBVar00144578NamePublic_namee2117
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00004541
LaboratoryCB
StatusLive
Affects (2)
DescriptionPhenotype (9)
Phenotype_not_observedWBPhenotype:0001732Paper_evidenceWBPaper00032033
Curator_confirmedWBPerson712
RemarkAnimals exhibited the same pH survival profile as the wild-type strain over a range of pH 3 to pH 10.Paper_evidenceWBPaper00032033
Curator_confirmedWBPerson712
Phenotype_assayTreatmentMixed or synchronised populations of nematodes were exposed to 500ul M9 buffer (100 mM phosphate, 85 mM NaCl, 1 mM MgSO4 buffered at the appropriate pH by varying KH2PO4 and Na2HPO4 concentrations accordingly) at varying pHs in a 24-well plate format for 4 h (200 nematodes/well). Nematodes were scored as viable by the presence of touch response and pharyngeal pumping.Paper_evidenceWBPaper00032033
Curator_confirmedWBPerson712
ReferenceWBPaper00005704
WBPaper00005747
WBPaper00032033
WBPaper00003348
WBPaper00000927
WBPaper00013885
MethodAllele