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WormBase Tree Display for Variation: WBVar00093800

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Name Class

WBVar00093800NamePublic_nameok2696
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00032722
LaboratoryRB
KO_consortium_allele
StatusLive
AffectsGeneWBGene00012352
DescriptionPhenotypeWBPhenotype:0000016Paper_evidenceWBPaper00065161
Curator_confirmedWBPerson712
Remark"...we found that either of the dkf-1 mutations caused hypersensitivity to aldicarb at an early age (Figure 1C)."Paper_evidenceWBPaper00065161
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000064PATO:0000460Paper_evidenceWBPaper00065161
Curator_confirmedWBPerson712
WBPhenotype:0000017Paper_evidenceWBPaper00065161
Curator_confirmedWBPerson712
Remark"Aldicarb sensitivity is known to increase with age in worms (Mulcahy et al., 2013) and we confirmed that we could quantify an increase in sensitivity for Bristol N2 at day 5 (Figure 1D). Strikingly, by day 5 both of the dkf-1 mutant alleles demonstrated the opposite age-dependent effect to wild-types, becoming more resistant to aldicarb (Figure 1D)."Paper_evidenceWBPaper00065161
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000068PATO:0000460Paper_evidenceWBPaper00065161
Curator_confirmedWBPerson712
WBPhenotype:0000273Paper_evidenceWBPaper00065161
Curator_confirmedWBPerson712
RemarkIn contrast with previous reports (Feng et al., 2006), we found only marginal effects of the dkf-1 mutations on locomotion rate when assessed at a young age (day 1 of adulthood Figure 1A). The dkf-1 ok2696 mutation had no effect on rate of thrashing, whereas the tm4906 allele exhibited a small, but significant (~12%) decrease. A strong defect in locomotion, however, became evident with age. By day 5 of adulthood, an age-dependent decrease in locomotion for the Bristol N2 wild-type was minimal; however, there was a substantial, significant decrease in locomotion for both of the dkf-1 mutant alleles (Figure 1B). The ok2696 and the tm4906 alleles had 96% and 81% age-dependent reductions in thrashing in comparison to only 13% for Bristol N2 wild-types.Paper_evidenceWBPaper00065161
Curator_confirmedWBPerson712
ReferenceWBPaper00065161
MethodKO_consortium_allele