WormBase Tree Display for Variation: WBVar00093800
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WBVar00093800 | Name | Public_name | ok2696 | ||||||
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Sequence_details | SeqStatus | Pending_curation | |||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Strain | WBStrain00032722 | ||||||||
Laboratory | RB | ||||||||
KO_consortium_allele | |||||||||
Status | Live | ||||||||
Affects | Gene | WBGene00012352 | |||||||
Description | Phenotype | WBPhenotype:0000016 | Paper_evidence | WBPaper00065161 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | "...we found that either of the dkf-1 mutations caused hypersensitivity to aldicarb at an early age (Figure 1C)." | Paper_evidence | WBPaper00065161 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000064 | PATO:0000460 | Paper_evidence | WBPaper00065161 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000017 | Paper_evidence | WBPaper00065161 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | "Aldicarb sensitivity is known to increase with age in worms (Mulcahy et al., 2013) and we confirmed that we could quantify an increase in sensitivity for Bristol N2 at day 5 (Figure 1D). Strikingly, by day 5 both of the dkf-1 mutant alleles demonstrated the opposite age-dependent effect to wild-types, becoming more resistant to aldicarb (Figure 1D)." | Paper_evidence | WBPaper00065161 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000068 | PATO:0000460 | Paper_evidence | WBPaper00065161 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000273 | Paper_evidence | WBPaper00065161 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | In contrast with previous reports (Feng et al., 2006), we found only marginal effects of the dkf-1 mutations on locomotion rate when assessed at a young age (day 1 of adulthood Figure 1A). The dkf-1 ok2696 mutation had no effect on rate of thrashing, whereas the tm4906 allele exhibited a small, but significant (~12%) decrease. A strong defect in locomotion, however, became evident with age. By day 5 of adulthood, an age-dependent decrease in locomotion for the Bristol N2 wild-type was minimal; however, there was a substantial, significant decrease in locomotion for both of the dkf-1 mutant alleles (Figure 1B). The ok2696 and the tm4906 alleles had 96% and 81% age-dependent reductions in thrashing in comparison to only 13% for Bristol N2 wild-types. | Paper_evidence | WBPaper00065161 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Reference | WBPaper00065161 | ||||||||
Method | KO_consortium_allele |