WormBase Tree Display for Variation: WBVar00093740

WBVar00093740 Name: Public_name: ok2625
    Other_name: F49E10.3.1:c.96-1_*9del
    HGVSg: CHROMOSOME_X:g.5886077_5886624del
  Sequence_details: SMap: S_parent: Sequence: F49E10
    Flanking_sequences: tcctattttttctattttttatatttttca tgcaaacaccttgattaccaagaacaaaac
    Mapping_target: F49E10
    Type_of_mutation: Deletion
    PCR_product: ok2625_external
      ok2625_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00032674
    Laboratory: RB
    Person: WBPerson46
    KO_consortium_allele
    Status: Live
  Affects: Gene: WBGene00001450
    Transcript: F49E10.3.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,3_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: F49E10.3.1:c.96-1_*9del
        cDNA_position: ?-545
        Intron_number: 2-4/5
        Exon_number: 3-6/6
  Isolation: Mutagen: EMS
  Description: Phenotype: WBPhenotype:0000542 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002286 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002287 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002292 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002301 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0002312 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0004016 Paper_evidence: WBPaper00064746
        Curator_confirmed: WBPerson40249
      WBPhenotype:0004022 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
      WBPhenotype:0004023 Paper_evidence: WBPaper00043908
        Curator_confirmed: WBPerson2987
    Phenotype_not_observed: WBPhenotype:0001524 Paper_evidence: WBPaper00036308
        Curator_confirmed: WBPerson2987
        Remark: 0% of flp-7(ok2625) animals were resistant to EGF-induced sleep (Table 1) Paper_evidence: WBPaper00036308
            Curator_confirmed: WBPerson2987
        Phenotype_assay: Treatment: Animals carrying the hs:LIN-3 transgene were well fed and grown at 20C. Young adult animals were scored 2 hours after heat shock for EGF-induced sleep behavior (see Materials and methods). Paper_evidence: WBPaper00036308
              Curator_confirmed: WBPerson2987
          Genotype: hs:LIN-3 Paper_evidence: WBPaper00036308
              Curator_confirmed: WBPerson2987
      WBPhenotype:0002432 Paper_evidence: WBPaper00050011
        Curator_confirmed: WBPerson712
        Remark: Null mutation of this neuropeptide had little or no effect on stress-induced sleep. To determine whether ALA-enriched neuropeptides are necessary for stress-induced sleep, we assayed locomotion, head movement, pharyngeal pumping, avoidance response, and defecation before and 30 min after heat shock. Single-null mutants were indistinguishable from wild-type with respect to pumping, locomotion, and head movement after heat shock. Paper_evidence: WBPaper00050011
            Curator_confirmed: WBPerson712
  Reference: WBPaper00043908
    WBPaper00036308
    WBPaper00050011
    WBPaper00064746
  Remark: Sequenced by the C. elegans Gene Knockout Consortium Paper_evidence: WBPaper00041807
  Method: KO_consortium_allele