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WormBase Tree Display for Variation: WBVar00091291

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Name Class

WBVar00091291EvidencePaper_evidenceWBPaper00032966
NamePublic_namenk3
Other_namekk3Paper_evidenceWBPaper00003454
HGVSgCHROMOSOME_V:g.6754312_6759906del
Sequence_detailsSMapS_parentSequenceT25F10
Flanking_sequencestcctgatcttgacgcggagtctgcgcctccgacatgcgggttgaatcttgcgggtgccgg
Mapping_targetT25F10
Type_of_mutationDeletion
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00007207
WBStrain00007209
WBStrain00029114
LaboratoryNU
StatusLive
AffectsGeneWBGene00000936
WBGene00195421
WBGene00198350
TranscriptT25F10.9VEP_consequencetranscript_ablation
VEP_impactHIGH
Exon_number1/1
T25F10.7VEP_consequencetranscript_ablation
VEP_impactHIGH
Exon_number1/1
T25F10.2.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
VEP_impactHIGH
cDNA_position?-1215
CDS_position?-1065
Protein_position?-355
Intron_number2-8/9
Exon_number1-9/10
InteractorWBInteraction000009115
WBInteraction000501769
WBInteraction000523983
WBInteraction000536169
GeneticsInterpolated_map_positionV0.136731
DescriptionPhenotypeWBPhenotype:0000062Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
RemarkWhile the wild-type population on S. marcescens survived until all animals ceased laying eggs, all dbl-1(nk3) animals died on S. marcescens by Day 5.Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
TreatmentAnimals were age-synchronized by hypochlorite treatment (Stiernagle, 2006) and grown on plates seeded with Escherichia coli OP50. Ten L4 animals were manually transferred to individual plates seeded with S. marcescens. Plates were completely covered by bacteria to prevent animals from avoiding the bacteria. Adults were daily transferred to new plates and the number of eggs laid on each plate was counted every 24 hours until no more eggs were laid. Statistical analyses were performed using repeated measures ANOVA and Tukey's post-hoc test. S. marcescens from Carolina Biological Supply Company.Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
GenotypeNU3Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
WBPhenotype:0001013Paper_evidenceWBPaper00005382
Curator_confirmedWBPerson712
Remarkdbl-1 mutants exhibited a dramatically reduced survival in the presence of Db11 and Db1140 relative to wild-type worms.Mutants showed reduced longevity when grown on OP50. Lifespan increased significantly in the presence of heat-killed OP50 and OP50 plates containing an inhibitor of bacterial replication.Paper_evidenceWBPaper00005382
Curator_confirmedWBPerson712
WBPhenotype:0001014Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
RemarkThis mutant is more resistant to enterotoxigenic Escherichia coli infection compared with N2 wild-type animals.Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
Phenotype_assayTreatmentAnimals grown on plates containing K88plus enterotoxigenic Escherichia coli (ETEC) strain JG280.Paper_evidenceWBPaper00055320
Curator_confirmedWBPerson557
WBPhenotype:0002544Paper_evidenceWBPaper00056139
Curator_confirmedWBPerson3878
RemarkNormally beneficial Enterobacter cloacae commensal - providing protection from a subsequent Enterococcus faecalis infection turn to pathogenicPaper_evidenceWBPaper00056139
Curator_confirmedWBPerson3878
WBPhenotype:0002545Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
Remarkdbl-1 mutant animals laid even fewer eggs than the wild-type animals on S. marcescens, suggesting that the reduced brood size phenotype is independently affected by both S. marcescens exposure and by loss of DBL-1 (p< 0.001).; While the decrease in brood size of wild-type animals on S. epidermidis was not significant (p= 0.115), the decreased brood size of dbl-1(nk3) animals was significant on this pathogenic bacterial strain (p= 0.045).; Wild-type and dbl-1(nk3) animals both significantly decrease their brood size when grown on S. marcescens (p= 0.005 and p< 0.001, respectively). dbl-1 mutant animals laid even fewer eggs than the wild-type animals on S. marcescens, suggesting that the reduced brood size phenotype is independently affected by both S. marcescens exposure and by loss of DBL-1 (p< 0.001).Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
TreatmentAnimals were age-synchronized by hypochlorite treatment (Stiernagle, 2006) and grown on plates seeded with Escherichia coli OP50. Ten L4 animals were manually transferred to individual plates seeded with S. marcescens. Plates were completely covered by bacteria to prevent animals from avoiding the bacteria. Adults were daily transferred to new plates and the number of eggs laid on each plate was counted every 24 hours until no more eggs were laid. Statistical analyses were performed using repeated measures ANOVA and Tukey's post-hoc test. S. marcescens from Carolina Biological Supply Company.Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
GenotypeNU3Paper_evidenceWBPaper00056577
Curator_confirmedWBPerson712
WBPhenotype:0002547Paper_evidenceWBPaper00056139
Curator_confirmedWBPerson3878
RemarkMutant animals exhibit a greater abundance of bacteria of the Enterobacteriaceae family in the gut, compared to controls.Paper_evidenceWBPaper00056139
Curator_confirmedWBPerson3878
Phenotype_not_observedWBPhenotype:0000114Paper_evidenceWBPaper00035891
Curator_confirmedWBPerson2987
Remark"We also tested the role of several other pathways implicated in C. elegans immunity, including the TGF-beta pathway, JNK pathway, the G protein-coupled receptor fshr-1, and the betacatenin homolog bar-1. None of the pathways we tested appeared to be required for induction of irg-1 (see SI Text and Fig S1)."Paper_evidenceWBPaper00035891
Curator_confirmedWBPerson2987
WBPhenotype:0001012Paper_evidenceWBPaper00049281
Curator_confirmedWBPerson33350
RemarkShorter lifespan when exposed to Mycobacterium marinum, similar to wild-type C. elegans. (Supplemental Figure 11A). Not involved in C. elegans innate immune response against mycobacteria.Paper_evidenceWBPaper00049281
Curator_confirmedWBPerson33350
Increased bagging when exposed to Mycobacterium marinum (~55%), similar to wild-type C. elegans. (Supplemental Figure 11B). Not involved in C. elegans innate immune response against mycobacteria.Paper_evidenceWBPaper00049281
Curator_confirmedWBPerson33350
Increased depigmentation when exposed to Mycobacterium marinum (~30%), similar to wild-type C. elegans. (Supplemental Figure 11C). Not involved in C. elegans innate immune response against mycobacteria.Paper_evidenceWBPaper00049281
Curator_confirmedWBPerson33350
Shorter length when exposed to Mycobacterium marinum (~15%), similar to wild-type C. elegans. (Supplemental Figure 11D). Not involved in C. elegans innate immune response against mycobacteria.Paper_evidenceWBPaper00049281
Curator_confirmedWBPerson33350
EQ_annotationsGO_termGO:0043473PATO:0000460Paper_evidenceWBPaper00049281
Curator_confirmedWBPerson33350
GO:0010171PATO:0000460Paper_evidenceWBPaper00049281
Curator_confirmedWBPerson33350
WBPhenotype:0001013Paper_evidenceWBPaper00049281
Curator_confirmedWBPerson33350
RemarkNo increased susceptibility to Mycobacterium marinum exposure compared to wild-type C. elegans. (Supplemental Figure 9A,C). Not involved in C. elegans innate immune response against mycobacteria.Paper_evidenceWBPaper00049281
Curator_confirmedWBPerson33350
WBPhenotype:0001184Paper_evidenceWBPaper00032966
Curator_confirmedWBPerson2021
RemarkAbnormally sized mutants did not display enhanced fat accumulationPaper_evidenceWBPaper00032966
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentNile Red stainingPaper_evidenceWBPaper00032966
Curator_confirmedWBPerson2021
Reference (11)
MethodDeletion_allele