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WormBase Tree Display for Variation: WBVar00090860

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Name Class

WBVar00090860EvidencePaper_evidenceWBPaper00031335
NamePublic_namen4541
Sequence_detailsSMapS_parentSequenceC39D10
Flanking_sequencesTTGTTGGAGAAATGAATAAATCTACAAAATTAGGGAACA
Mapping_targetC39D10
Type_of_mutationDeletion
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00027532
WBStrain00027588
LaboratoryMT
StatusLive
AffectsGeneWBGene00255565
WBGene00003334
WBGene00045343
TranscriptC39D10.18
C39D10.12
C39D10.10
InteractorWBInteraction000576759
WBInteraction000576760
WBInteraction000576761
WBInteraction000576762
WBInteraction000576763
WBInteraction000576764
WBInteraction000576765
WBInteraction000576766
WBInteraction000576767
IsolationMutagenEMSPaper_evidenceWBPaper00031335
GeneticsInterpolated_map_positionX-1.03875
DescriptionPhenotypeWBPhenotype:0000157Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
RemarkThe posterior contraction in n4541 mutant worms often appeared to be biphasic, with an initial weak contraction, followed by a full contraction. This was associated with a longer interval between the posterior contraction and subsequent steps in the motor program.Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
The posterior contraction in n4541 mutant worms often appeared to be weak and were not associated with a full DMP (defecation motor program). This occurred in 34.6 percent of the worms.Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
PenetranceLow34.6 percentPaper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
WBPhenotype:0000205Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
Remarkn4541 mutant worms occasionally failed to execute an enteric muscle contraction and expulsion following a strong posterior body-contraction event. This occurred in 14.8 percent cycles.Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
PenetranceLowOccurred in 14.8 percent of the cycles observed.Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
WBPhenotype:0000208Paper_evidenceWBPaper00031335
WBPaper00041724
Curator_confirmedWBPerson2021
WBPerson557
RemarkLength of defecation cycle is increasedPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
n4541 worms display long, arrhythmic defecation cycles, as determined by the length of time between consecutive posterior body-contraction events. The n4541 allele deletes the mir-240/786 micro-RNA cluster, but it was determined through rescue experiments that it was loss of the mir-786 region that causes the phenotype, not the mir-240 region.Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0000650Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
Remarkn4541 worms display long, arrhythmic defecation cycles, as determined by the length of time between consecutive posterior body-contraction events. The n4541 allele deletes the mir-240/786 micro-RNA cluster, but it was determined through rescue experiments that it was loss of the mir-786 region that causes the phenotype, not the mir-240 region.Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
WBPhenotype:0000994Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
Remarkn4541 mutant worms occasionally failed to execute an enteric muscle contraction and expulsion following a strong posterior body-contraction event. This occurred in 14.8 percent of the cycles.Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
PenetranceLowOccurred in 14.8 percent of the cycles observed.Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
WBPhenotype:0001347Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
RemarkCauses ectopic intestinal calcium-wave initiation. In wild-type worms, each calcium peak represents a fast intercellular calcium wave that initiates in the posterior intestine and propagates anteriorly. Multiple defects in calcium oscillations were observed in mir-240/786(n4541) mutants. First, calcium oscillations were arrhythmic and differed greatly in magnitude in mir-240/786cmutants (Figure 4C) relative to wild-type controls (Figure 4A). Second, multiple calcium events occurred in each defecation cycle (Figure 4H), with small calcium increases often preceding successively larger calcium increases, until the DMP was triggered (Figures 4C and S1). Third, the spatial pattern of calcium-wave initiation was strikingly altered in mir-240/786 mutants. Whereas, in wild-type worms, calcium-wave initiation is restricted to the anterior and posterior ends of the intestine, calcium-wave initiation in mir-240/786 mutants often occurred at ectopic sites in internal intestinal cells.Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
Phenotype_assayGenotypepha-1(e2123ts) III; him-5(e1490) V; mir-240/786(n4541) X; rnyEx109 [Pnhx-2::D3cpv +pha-1(+)].Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
WBPhenotype:0001640Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
RemarkAcidification of the intestinal cytoplasm results from calcium signaling during defecation and contributes to the behavioral output. In mir-240/786(n4541) worms acidification events were arrhythmic.Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
Phenotype_assayGenotypepha-1(e2123ts) III; mir-240/786 (n4541) X; rnyEx006 [Pnhx-2::pHluorin, pha-1(+)]Paper_evidenceWBPaper00041724
Curator_confirmedWBPerson557
Phenotype_not_observedWBPhenotype:0000006Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Remarknon-EglPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0000634Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
RemarkPumping is normalPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0000637Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
RemarkNo defects in dauer formationPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0000643Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Remarknon-UncPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0001233Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
RemarkCell number and nuclear staining is normalPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentDAPI stainingPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBPhenotype:0002535Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Remarknon-DyfPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0005666PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0005667PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0005668PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0005665PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0005661PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0007807PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
WBbt:0007808PATO:0000460Paper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentDiO stainingPaper_evidenceWBPaper00031335
Curator_confirmedWBPerson2021
ReferenceWBPaper00031335
WBPaper00041724
MethodDeletion_allele