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WormBase Tree Display for Variation: WBVar00089074

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Name Class

WBVar00089074NamePublic_namemn158
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00034249
LaboratorySP
StatusLive
AffectsGeneWBGene00004291
InteractorWBInteraction000052108
WBInteraction000052426
WBInteraction000052427
WBInteraction000052428
WBInteraction000052429
WBInteraction000052430
WBInteraction000052431
GeneticsMapping_dataIn_multi_point367
DescriptionPhenotypeWBPhenotype:0000050Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkLess than 25% eggs hatch at 15C, whereas over 90% eggs hatched at 25C. There was no temperature sensitive period for this embryonic lethality; however, the egg-hatching frequency at 15C decreased as parental age increased, 44.7% (268/600) - 3.2% (4/122) from 1 - 8 day old adults respectively.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Temperature_sensitiveCold_sensitive15CPaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
MaternalWith_maternal_effectPaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000154Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkReduced brood size (34% of wildtype at 20C); < 25% eggs hatch at 15C.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveCold_sensitivePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Phenotype_assayTemperature20CPaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Ease_of_scoringES1_Very_hard_to_scorePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000275Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkAnimals exhibit moderate levels of UV hypersensitivity versus extreme hypersensitivity.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
hypersensitive to UVPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Temperature_sensitiveCold_sensitivePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Phenotype_assayTreatmentUV sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 1Jm 10X-2/sec. Irradiated animals were protected from light. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000742Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkAnimals show slight but statistically significant deviations from the wild-type recombination frequency between the dpy-7 and unc-3 interval.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayTreatmentRecombination frequencies were measured using unlinked intervals, dpy-10 unc-4 or dpy-7 unc-3.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000784Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkMale fertility was 16% that of wild type.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0001664Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarkreduced X chromosome nondisjunction (partly suppresses some him mutations)Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Temperature_sensitiveCold_sensitivePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001665Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkAnimals have lower levels of X-chromosome nondisjunction as measured by fewer instances of male self-progeny (0.03%, n=9689 animals, 90 broods) compared to wild-type (0.32% males, n=5542 animals, 20 broods).Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0001667Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkAnimals are hypersensitive to acute exposure to MMS but not to chronic exposure.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Hypersensitive to acute MMS treatmentPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Temperature_sensitiveCold_sensitivePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were tested in two chronic and 1 acute drug exposure assays: 1. gravid adults were placed on plates containing various concentrations of MMS and allowed to develop; 2. L4 larvae were incubated on plates containing various concentrations of MMS for 24 hours, their progeny were scored for predominant stage of developmental arrest after further incubation; 3. acute exposure was assayed by incubating eggs in 50mM MMS for various times before plating and ounting egg hatch and survival to adulthood.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000228Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkNo non-Unc revertants of unc-58 were observed on 24 plates.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Phenotype_assayGenotypeunc-58(e665)Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000276Paper_evidenceWBPaper00000565
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarknot hypersensitive to X irradiationPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
Temperature_sensitiveCold_sensitivePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
Phenotype_assayTreatmentX-ray sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 470 r/min. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000392Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0000520Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkAnimals were indistinguishable in appearance from wild type.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
WBPhenotype:0001631Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
RemarkMales produced equal numbers of X-bearing and nullo-X-bearing sperm.Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000565
Curator_confirmedWBPerson712
ReferenceWBPaper00000565
WBPaper00016084
MethodAllele