WormBase Tree Display for Variation: WBVar00089074
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WBVar00089074 | Name | Public_name | mn158 | ||||||
---|---|---|---|---|---|---|---|---|---|
Sequence_details | SeqStatus | Pending_curation | |||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Strain | WBStrain00034249 | ||||||||
Laboratory | SP | ||||||||
Status | Live | ||||||||
Affects | Gene | WBGene00004291 | |||||||
Interactor | WBInteraction000052108 | ||||||||
WBInteraction000052426 | |||||||||
WBInteraction000052427 | |||||||||
WBInteraction000052428 | |||||||||
WBInteraction000052429 | |||||||||
WBInteraction000052430 | |||||||||
WBInteraction000052431 | |||||||||
Genetics | Mapping_data | In_multi_point | 367 | ||||||
Description | Phenotype | WBPhenotype:0000050 | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Less than 25% eggs hatch at 15C, whereas over 90% eggs hatched at 25C. There was no temperature sensitive period for this embryonic lethality; however, the egg-hatching frequency at 15C decreased as parental age increased, 44.7% (268/600) - 3.2% (4/122) from 1 - 8 day old adults respectively. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Cold_sensitive | 15C | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Maternal | With_maternal_effect | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000154 | Paper_evidence | WBPaper00000565 | |||||||
Person_evidence | WBPerson261 | ||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Reduced brood size (34% of wildtype at 20C); < 25% eggs hatch at 15C. | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Cold_sensitive | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Temperature | 20C | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Ease_of_scoring | ES1_Very_hard_to_score | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000275 | Paper_evidence | WBPaper00000565 | |||||||
Person_evidence | WBPerson261 | ||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals exhibit moderate levels of UV hypersensitivity versus extreme hypersensitivity. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
hypersensitive to UV | Person_evidence | WBPerson261 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Cold_sensitive | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | UV sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 1Jm 10X-2/sec. Irradiated animals were protected from light. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls. | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000742 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals show slight but statistically significant deviations from the wild-type recombination frequency between the dpy-7 and unc-3 interval. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Recombination frequencies were measured using unlinked intervals, dpy-10 unc-4 or dpy-7 unc-3. | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000784 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Male fertility was 16% that of wild type. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001664 | Person_evidence | WBPerson261 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | reduced X chromosome nondisjunction (partly suppresses some him mutations) | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Cold_sensitive | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001665 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals have lower levels of X-chromosome nondisjunction as measured by fewer instances of male self-progeny (0.03%, n=9689 animals, 90 broods) compared to wild-type (0.32% males, n=5542 animals, 20 broods). | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001667 | Paper_evidence | WBPaper00000565 | |||||||
Person_evidence | WBPerson261 | ||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals are hypersensitive to acute exposure to MMS but not to chronic exposure. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Hypersensitive to acute MMS treatment | Person_evidence | WBPerson261 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Cold_sensitive | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Animals were tested in two chronic and 1 acute drug exposure assays: 1. gravid adults were placed on plates containing various concentrations of MMS and allowed to develop; 2. L4 larvae were incubated on plates containing various concentrations of MMS for 24 hours, their progeny were scored for predominant stage of developmental arrest after further incubation; 3. acute exposure was assayed by incubating eggs in 50mM MMS for various times before plating and ounting egg hatch and survival to adulthood. | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_not_observed | WBPhenotype:0000228 | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | No non-Unc revertants of unc-58 were observed on 24 plates. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Genotype | unc-58(e665) | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000276 | Paper_evidence | WBPaper00000565 | |||||||
Person_evidence | WBPerson261 | ||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | not hypersensitive to X irradiation | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Cold_sensitive | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | X-ray sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 470 r/min. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls. | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000392 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000520 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals were indistinguishable in appearance from wild type. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001631 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Males produced equal numbers of X-bearing and nullo-X-bearing sperm. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000056 | PATO:0000460 | Paper_evidence | WBPaper00000565 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Reference | WBPaper00000565 | ||||||||
WBPaper00016084 | |||||||||
Method | Allele |